Detection of Glucose and Acid Phosphatase Using a Multifunctional Enzyme Cascade Sensing System Based on Hg<sub>2</sub>［Fe（CN）<sub>6</sub>］ Nanozyme

GUO Ao-huan; LI Ping; WANG Guang-li

doi:10.19969/j.fxcsxb.22100601

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Detection of Glucose and Acid Phosphatase Using a Multifunctional Enzyme Cascade Sensing System Based on Hg₂［Fe（CN）₆］ Nanozyme

Experimental Techniques and Methods | 更新时间：2023-04-18

- Detection of Glucose and Acid Phosphatase Using a Multifunctional Enzyme Cascade Sensing System Based on Hg₂［Fe（CN）₆］ Nanozyme
- Journal of Instrumental Analysis Vol. 42, Issue 4, Pages: 488-494(2023)
- 作者机构：
  
  江南大学化学与材料工程学院，江苏无锡 214122
- 作者简介：
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- DOI：10.19969/j.fxcsxb.22100601
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郭澳环,李苹,王光丽.基于Hg₂［Fe（CN）₆］纳米酶的多功能酶级联传感体系检测葡萄糖和酸性磷酸酶[J].分析测试学报,2023,42(04):488-494.

GUO Ao-huan,LI Ping,WANG Guang-li.Detection of Glucose and Acid Phosphatase Using a Multifunctional Enzyme Cascade Sensing System Based on Hg₂［Fe（CN）₆］ Nanozyme[J].Journal of Instrumental Analysis,2023,42(04):488-494.
郭澳环,李苹,王光丽.基于Hg₂［Fe（CN）₆］纳米酶的多功能酶级联传感体系检测葡萄糖和酸性磷酸酶[J].分析测试学报,2023,42(04):488-494. DOI： 10.19969/j.fxcsxb.22100601.

GUO Ao-huan,LI Ping,WANG Guang-li.Detection of Glucose and Acid Phosphatase Using a Multifunctional Enzyme Cascade Sensing System Based on Hg₂［Fe（CN）₆］ Nanozyme[J].Journal of Instrumental Analysis,2023,42(04):488-494. DOI： 10.19969/j.fxcsxb.22100601.

摘要

该文基于Hg,2+,可与K,4,［Fe（CN）,6,］原位反应形成Hg,2,［Fe（CN）,6,］纳米酶的机理，并利用过氧化氢（H,2,O,2,）可激发Hg,2,［Fe（CN）,6,］酶活性，以及焦磷酸盐（PPi）可通过与Hg,2+,结合阻止Hg,2,［Fe（CN）,6,］纳米酶的形成的原理，构建了多功能酶级联传感平台。当葡萄糖存在时，葡萄糖氧化酶氧化葡萄糖生成H,2,O,2,，Hg,2,［Fe（CN）,6,］催化H,2,O,2,分解产生的羟基自由基（·OH）可氧化底物3，3'，5，5'-四甲基联苯胺（TMB）显色，从而实现葡萄糖的检测。该方法对葡萄糖检测的线性范围为0.10 ~ 500 μmol/L，检出限为30 nmol/L。当酸性磷酸酶（ACP）存在时，ACP可以水解PPi并释放出与PPi结合的Hg,2+,，Hg,2+,与K,4,［Fe（CN）,6,］原位反应生成Hg,2,［Fe（CN）,6,］纳米酶，进而可实现ACP的检测。结果表明，该方法对ACP检测的线性范围为0.03 ~ 50 U/L，检出限为0.007 0 U/L。该传感平台操作简单、灵敏度高，为多功能传感体系的设计提供了一种新的思路。

Abstract

According to the formation of Hg,2,［Fe（CN）,6,］ nanozyme based on the in-situ reaction of Hg,2+, and K,4,［Fe（CN）,6,］，a multifunctional biosensing platform was constructed based on an enzyme cascade in which the activity of Hg,2,［Fe（CN）,6,］ depended on hydrogen peroxide（H,2,O,2,），as well as pyrophosphate（PPi） was able to bind to Hg,2+, and prevent the formation of Hg,2,［Fe（CN）,6,］ nanozyme．In the presence of glucose，glucose oxidase oxidized glucose to generate H,2,O,2,，and then Hg,2,［Fe（CN）,6,］ catalyzed the decomposition of H,2,O,2, to produce hydroxyl radicals（·OH） to oxidize the substrate of 3，3'，5，5'-tetramethylbenzidine（TMB），which enabled the detection of glucose．The linear range of the method for glucose detection was 0.10－500 μmol/L，and the detection limit was 30 nmol/L．In the presence of acid phosphatase（ACP），ACP could hydrolyze PPi to release Hg,2+, that could otherwise be bound by PPi，which allowed the reaction of K,4,［Fe（CN）,6,］ with Hg,2+, to generate Hg,2,［Fe（CN）,6,］ nanozyme for catalyzing the oxidation of TMB．Results showed that the linear range of the method for the detection of ACP was 0.03－50 U/L，and the detection limit was 0.007 0 U/L．This sensing platform is different from the traditional detection system for single target，which provides a new idea for the design of multifunctional sensing systems with simple operation and high sensitivity.

关键词

纳米酶酶级联体系Hg2［Fe（CN）6］葡萄糖酸性磷酸酶（ACP）

Keywords

nanozymeenzyme cascade systemHg2［Fe（CN）6］glucoseacid phosphatase（ACP）

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Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology，Colleges and Universities Key Laboratory for Efficient Use of Agricultural Resources in the Southeast of Guangxi，College of Chemistry and Food Science，Yulin Normal University

College of Chemical Engineering，Fuzhou University

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Shanghai Key Laboratory of Functional Materials Chemistry，School of Chemistry and Molecular Engineering， East China University of Science and Technology

High & New Technology Research Center of Henan Academy of Science

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Detection of Glucose and Acid Phosphatase Using a Multifunctional Enzyme Cascade Sensing System Based on Hg2［Fe（CN）6］ Nanozyme

DOI：10.19969/j.fxcsxb.22100601

摘要

Abstract

关键词

Keywords

references

Detection of Glucose and Acid Phosphatase Using a Multifunctional Enzyme Cascade Sensing System Based on Hg₂［Fe（CN）₆］ Nanozyme