Preparation of a Laser Induced Graphene/Enzyme Electrode and Its Application in Glucose Detection

CHEN Fang-ru; ZHANG Zhao-kang; HE Shu-han; YU Xi-xi; LI Yan-xia

doi:10.19969/j.fxcsxb.22082503

您当前的位置：

首页 >

文章列表页 >

Preparation of a Laser Induced Graphene/Enzyme Electrode and Its Application in Glucose Detection

Scientific Papers | 更新时间：2023-02-28

- Preparation of a Laser Induced Graphene/Enzyme Electrode and Its Application in Glucose Detection
- Journal of Instrumental Analysis Vol. 42, Issue 2, Pages: 150-157(2023)
- 作者机构：
  
  1.闽江学院材料与化学工程学院，福建福州 350108
  2.福州大学石油化工学院，福建福州 350108
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.22082503
  CLC：
扫描看全文
陈芳如,张兆康,何书涵等.激光诱导石墨烯/酶电极的葡萄糖传感研究[J].分析测试学报,2023,42(02):150-157.

CHEN Fang-ru,ZHANG Zhao-kang,HE Shu-han,et al.Preparation of a Laser Induced Graphene/Enzyme Electrode and Its Application in Glucose Detection[J].Journal of Instrumental Analysis,2023,42(02):150-157.
陈芳如,张兆康,何书涵等.激光诱导石墨烯/酶电极的葡萄糖传感研究[J].分析测试学报,2023,42(02):150-157. DOI： 10.19969/j.fxcsxb.22082503.

CHEN Fang-ru,ZHANG Zhao-kang,HE Shu-han,et al.Preparation of a Laser Induced Graphene/Enzyme Electrode and Its Application in Glucose Detection[J].Journal of Instrumental Analysis,2023,42(02):150-157. DOI： 10.19969/j.fxcsxb.22082503.

摘要

葡萄糖水平是糖尿病诊断治疗的一项重要评价指标。该文基于激光诱导石墨烯/酶电极构建了一种新型便携式葡萄糖传感器。通过激光诱导石墨烯（LIG）技术制备微型柔性石墨烯三电极系统（LIG电极），并基于1-芘丁酸的疏水端苯环与石墨烯的六元环结构间的π－π叠加效应，对LIG电极表面羧基化，进而共价交联葡萄糖氧化酶（GOx），制得LIG/GOx电极，采用计时电流法，开展葡萄糖传感研究。结果表明，所构建的传感器对葡萄糖的检测范围为5.0 × 10,-5, ~ 1.0 × 10,-2, mol/L，检出限（,S,/,N, = 2）为5.0 × 10,-5, mol/L。方法表现出较高的选择性、稳定性和良好的重复性，可用于血清、尿样和汗液中葡萄糖的检测。LIG在葡萄糖传感微型电极材料的应用，有利于实现葡萄糖的非临床检测，有望用于便携式传感器的构建。

Abstract

Glucose level is an important evaluation index for the diagnosis and treatment of diabetes．In this paper，a novel portable glucose sensor was constructed based on laser-induced graphene/enzyme electrodes.Micro-flexible graphene three-electrode system（LIG electrode） was prepared by laser-induced graphene（LIG） technique．The effect of π－π superposition occurred between the hydrophobic terminal benzene ring of 1-pyrene butyric acid and the six-membered ring structure of graphene introduced the carboxyl onto the LIG electrode，then the glucose oxidase（GOx） was covalently cross-linked to the surface of LIG for preparation of LIG/GOx electrodes．Glucose sensing was carried out by chronoamperometry．Results showed that the constructed sensor could be used to detect glucose in the range of 5.0 × 10,-5,－1.0 × 10,-2, mol/L with a limit of detectton（,S,/,N, = 2） of 5.0 × 10,-5, mol/L．The method showed high selectivity，stability and good reusability，and could be used to detect glucose in serum，urine and sweat samples．LIG is a micro-electrode material for glucose sensing with good development prospects，which is conducive to the realization of non-clinical glucose detection and is expected to be used in the construction of portable sensors.

关键词

激光诱导石墨烯葡萄糖电化学分析生物传感器

Keywords

laser induced grapheneglucoseelectrochemical analysisbiosensor

references

Liu H，Qiao J T，Fang S，Liu Y F，Li Q．J. Tianjin Polytech. Univ. （刘皓，乔巨涛，方纾，刘越峰，李倩．天津工业大学学报），2021，40（2）：46－54.

Teymourian H，Barfidokht A，Wang J．Chem. Soc. Rev.，2020，49：7671－7709.

Gao N，Wang S P，Liu X，Xia D C，Cai Z W，Chang G，He Y B．Chin. J. Anal. Chem. （高难，王帅澎，刘旭，夏大成，蔡志伟，常钢，何云斌．分析化学），2021，49（10）：1640－1648.

Hou T X．Application of Laser Interference and Laser Direct Writing Technology in the Fabrication of Controllable Micro Nano Structures. Beijing：Beijing Technology University（侯婷琇．激光干涉和激光直写技术应用于可控微纳结构的制备．北京：北京工业大学），2017.

Wang Q，Li C，Zhang Z X．J. Instrum. Anal. （王琪，李超，张召香．分析测试学报），2021，40（8）：1197－1202.

Cao H，Zhang H，Yang C，Jiang K，Wang J．J. Instrum. Anal. （曹慧，张慧，杨春，姜侃，王瑾．分析测试学报），2022，41（8）：1207－1223.

Rajaji U，Ganesh P S，Kim S Y，Govindasamy M，Alshgari R A，Liu T Y．ACS Appl. Nano Mater.，2022，5（3）：3252－3264.

Lu L S，Wang W T，Xie Y X，Tang Y．Chin. J. Mech. Eng. （陆龙生，王文涛，谢颖熙，汤勇．机械工程学报），2021，57（21）：234－247.

Zhao J，Zheng C，Gao J，Gui J H，Deng L C，Wang Y Y，Xu R Q．Sens. Actuators B，2021，347：130653.

Liu W，Huang Y H，Peng Y D，Walczak M，Wang D，Chen Q，Liu Z，Li L．ACS Appl. Nano Mater.，2020，3（1）：283－293.

Jayakumar K，Bennett R，Leech D．Electrochim. Acta，2021，371：137845.

Vivaldi F M，Dallinger A，Bonini A，Poma N，Sembranti L，Biagini D，Salvo P，Greco F，Di Francesco F．ACS Appl. Mater. Interfaces，2021，13（26）：30245－30260.

Lu Z W，Wu L，Dai X X，Wang Y Y，Sun M M，Zhou C L，Du H J，Rao H B．J. Hazard. Mater.，2020，402：123774.

Lin J，Peng Z W，Liu Y Y，Ruiz－Zepeda F，Ye R Q，Samuel E L G，Yacaman M J，Yakobson B I，Tour J M．Nat. Commun.，2014，5：1－8.

Luong D X，Subramanian A K，Silva G A L，Yoon J，Cofer S，Yang K，Owuor P S，Wang T，Wang Z，Lou J，Cofer S，Yang K，Owuor P S，Wang T，Wang Z，Ajayan P M，Tour J M．Adv. Mater.，2018，30（28）：1707416.

Fenzl C，Nayak P，Hirsch T，Wolfbeis O S，Alshareef H N，Baeumner A J．ACS Sens.，2017，2（5）：616－620.

Jamaluddin R A Z R，Tan L L，Chong K F，Heng L Y．Nanotechnology，2020，31（48）：5501.

Hossain M F，Slaughter G．Bioelectrochemistry，2021，141：107870.

Wang G Y，Chen J Y，Huang L，Chen Y T，Li Y X．Analyst，2021，146：6631－6642.

Mei Z F，Yao M T，Chen R S，Hu C G．J. Anal. Sci. （梅正方，姚梦婷，陈荣生，胡成国．分析科学学报），2021，37（4）：522－528.

He F，Qin X L，Fu Y C，Chen C，Xie Q J，Yao S Z．J. Electrochem. （何芳，覃晓丽，傅迎春，陈超，谢青季，姚守拙．电化学），2014，20（6）：515－520.

Wang Y，Xiang D，Ma S Q，Zhao L P，Li X Z．J. Shandong Jianzhu Univ. （王悦，项东，马绍群，赵丽萍，李秀泽．山东建筑大学学报），2022，37（2）：76－86.

Liu L，Zhang Z F，Wang S L，Li H F，Li K K，Ma L，Wei P Y，Zhang Y，Wu K N．Food Sci. 刘丽，张智锋，王松磊，李海峰，李恳恳，马亮，魏培媛，张也，武康宁．食品科学），2022，43（8）：339－345.

Zhu J，Liu S B，Hu Z H，Zhang X Z，Yi N，Tang K R，Dexheimer M G，Lian X J，Wang Q，Yang J，Gray J，Cheng H Y．Biosens. Bioelectron.，2021，193：113606.

Xu M Y，Zhu Y，Gao S H，Zhang Z X，Gu Y X，Liu X Y．ACS Appl. Nano Mater.，2021，4：12442－12452.

Lu Y，Wang W L．Appl. Electron. Tech. （路瑶，王文廉．电子技术应用），2020，46（6）：101－104.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Detection of Glucose Using an Electrochemiluminescence Biosensor Based on Ru（bpy）2+3-Triethylamine System

Detection of Glucose Using an Electrochemiluminescence Biosensor Based on Carboxyl Functionalized Polymer Dots

Fabrication of Glucose Biosensor Enhanced by Au Nanoparticles

Synthesis and Characterization of a Long-term Stable Lead-dependent DNAzyme

G-quadruple/Hemin DNAzyme-based Visual Detection of the Folding Structure Formed by d（GGA） Trinucleotide Repeats

Related Author

No data

Related Institution

重庆市沙坪坝区人民医院消化内科

西南大学化学化工学院

重庆市第八中学校

聊城大学化学化工学院

Hunan Provincial Key Laboratory of Water Treatment Functional Materials，Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology，College of Chemistry and Materials Engineering，Hunan University of Arts and Science

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰