Detection of NF－κB p50 Using a Biosensing Platform Based on In Situ Nanozyme on PbTiO<sub>3</sub> Surface

CHEN Yan-ru; ZHANG Lan; WANG Guang-li

doi:10.19969/j.fxcsxb.22050801

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Detection of NF－κB p50 Using a Biosensing Platform Based on In Situ Nanozyme on PbTiO₃ Surface

Scientific Papers | 更新时间：2023-01-04

- Detection of NF－κB p50 Using a Biosensing Platform Based on In Situ Nanozyme on PbTiO₃ Surface
- Journal of Instrumental Analysis Vol. 41, Issue 12, Pages: 1785-1792(2022)
- 作者机构：
  
  江南大学化学与材料工程学院，江苏无锡 214122
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.22050801
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陈彦如,张岚,王光丽.基于PbTiO₃表面原位纳米酶的生物传感平台用于NF－κB p50检测[J].分析测试学报,2022,41(12):1785-1792.

CHEN Yan-ru,ZHANG Lan,WANG Guang-li.Detection of NF－κB p50 Using a Biosensing Platform Based on In Situ Nanozyme on PbTiO₃ Surface[J].Journal of Instrumental Analysis,2022,41(12):1785-1792.
陈彦如,张岚,王光丽.基于PbTiO₃表面原位纳米酶的生物传感平台用于NF－κB p50检测[J].分析测试学报,2022,41(12):1785-1792. DOI： 10.19969/j.fxcsxb.22050801.

CHEN Yan-ru,ZHANG Lan,WANG Guang-li.Detection of NF－κB p50 Using a Biosensing Platform Based on In Situ Nanozyme on PbTiO₃ Surface[J].Journal of Instrumental Analysis,2022,41(12):1785-1792. DOI： 10.19969/j.fxcsxb.22050801.

摘要

该文介绍了一种通过将K,4,Fe（CN）,6,配位到PbTiO,3,表面获得原位纳米酶的新策略。利用脱氧核糖核苷5'-单磷酸（dNMP）在PbTiO,3,表面的结合，阻止K,4,Fe（CN）,6,在PbTiO,3,表面原位形成纳米酶，构建了基于纳米酶的生物传感平台。当NF－κB p50存在时，dNMP难以产生，K,4,Fe（CN）,6,得以顺利结合到PbTiO,3,表面，并在其表面自发形成纳米酶，催化TMB氧化以定量检测NF－κB p50。实验表明，该方法对NF－κB p50的检测线性范围为3.0 pmol/L ~ 10 nmol/L，检出限（,S,/,N, = 3）为1.2 pmol/L，对实际样品的加标回收率为99.1% ~ 102%，相对标准偏差不大于5.3%。该方法为NF－κB p50的检测提供了一种无标记、无固定且具有信号放大作用的新策略，不仅灵敏度高、选择性好、操作简便，且在实际样品检测中具有潜在的应用价值。

Abstract

A biosensing platform for the detection of NF－κB p50 was constructed，based on an ,in situ, nanozyme with adjustable catalytic activity．The ,in situ, nanozyme was obtained by combining K,4,Fe（CN）,6, onto the surface of PbTiO,3,．Generally，the attachment of deoxyribonucleoside 5'-monophosphate（dNMP） onto the PbTiO,3, surface may prevent the spontaneous formation of nanozyme on its surface，while in the presence of NF－κB p50，dNMP is hardly to be generated，which allows K,4,Fe（CN）,6, combining smoothly to the surface of PbTiO,3,，and a spontaneously formed nanozyme on PbTiO,3, surface catalyzes TMB oxidation for the quantitative detection of NF－κB p50．The results showed that the detection linear range of this method for NF－κB p50 was 3.0 pmol/L－10 nmol/L，while the limits of detection（,S,/,N ,= 3） was 1.2 pmol/L．The spiked recoveries for real samples ranged from 99.1% to 102%，with relative standard deviations not more than 5.3%．This method provides a new strategy in label-free，immobilization-free mode for the detection of NF－κB p50 with amplified signal，which not only has high sensitivity，good selectivity and simple operation，but also has an application potential in the detection of actual samples.

关键词

纳米酶生物传感PbTiO3NF－κB p50

Keywords

nanozymebiosensingPbTiO3NF－κB p50

references

Fan Z Q，Wang J F，Hao N，Li Y H，Yin Y H，Wang Z P，Ding Y D，Zhao J F，Zhang K，Huang W．Chem. Commun.，2019，55（79）：11892－11895.

Lu L H，Su H J，Li F．Anal. Chem.，2017，89（16）：8328－8334.

Li B Z，Xu L，Chen Y，Zhu W Y，Shen X，Zhu C H，Luo J P，Li X X，Hong J L，Zhou X M．Anal. Chem.，2017，89（14）：7316－7323.

Zhang Y，Hu J，Zhang C Y．Anal. Chem.，2012，84（21）：9544－9549.

Huang Y Y，Ren J S，Qu X G．Chem. Rev.，2019，119（6）：4357－4412.

Wang P Y，Zhang L Y，Zhang W B．J. Instrum. Anal. （王佩瑶，张凌怡，张维冰．分析测试学报），2022，41（8）：1121－1129.

Jin L Y，Dong Y M，Wu X M，Cao G X，Wang G L．Anal. Chem.，2015，87（20）：10429－10436.

Hamid K，Ali S N，Mohammad E A，Farhad A，Vahid A，Mehdi R N．Sep. Purif. Technol.，2019，211（18）：873－881.

Sha L，Zhang X J，Wang G F．Biosens. Bioelectron.，2016，82（15）：85－92.

Khoudiakov M，Parise A R，Brunschwig B S．J. Am. Chem. Soc.，2003，125（15）：4637－4642.

Kanagadurai R，Sankar R，Sivanesan G，Srinivasan S，Jayavel R．Cryst. Res. Technol.，2007，41（9）：853－858.

Jiang X Q，Wang X Y，Lin A Q，Wei H．Anal. Chem.，2021，93（14）：5954－5962.

Gao L Z，Zhuang J，Nie L，Zhang J B，Zhang Y，Gu N，Wang T H，Feng J，Yang D L，Yan X Y．Nat. Nanotechnol.，2007，2（9）：577－583.

An C H，Wang J Z，Qin C，Jiang W，Wang S T，Li Y，Zhang Q H．J. Mater. Chem.，2012，22（26）：13153－13158.

Zhao L，Wang J，Su D D，Zhang Y Y，Lu H Y，Yan X Y，Bai J H，Gao Y，Lu G Y．Nanoscale，2020，12（37）：19420－19428.

Wang W，Jiang X P，Chen K Z．Chem. Commun.，2012，48（58）：7289－7291.

Zhou S W，Sha H Z，Liu B R，Du X Z．Chem. Sci.，2014，5（11）：4424－4433.

Xu X Y，Zhao Z，Qin L D，Wei W，Levine J E，Mirkin C A．Anal. Chem.，2008，80（14）：5616－5621.

Zhang K，Fan Z Q，Yao B，Zhang T T，Ding Y D，Zhu S，Xie M H．Biosens. Bioelectron.，2021，176（15）：112942.

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Shanghai Key Laboratory of Functional Materials Chemistry，School of Chemistry and Molecular Engineering， East China University of Science and Technology

清华大学化学系

微量分析测试方法和仪器研制北京市重点实验室生命有机磷化学和化学生物学教育部重点实验室

东北大学理学院化学系

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Detection of NF－κB p50 Using a Biosensing Platform Based on In Situ Nanozyme on PbTiO3 Surface

DOI：10.19969/j.fxcsxb.22050801

摘要

Abstract

关键词

Keywords

references

Detection of NF－κB p50 Using a Biosensing Platform Based on In Situ Nanozyme on PbTiO₃ Surface