A Sandwich-type Electrochemical Immunosensor Based on Platinum Hybrid Multiwalled Carbon Nanotubes Adhered Copper Oxide

SUN Jing-bo; LUAN Ye-bo; MA Shi-hua; ZHENG Zhao; XING Yi-wen; ZHANG Hong-jing

doi:10.19969/j.fxcsxb.22050501

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A Sandwich-type Electrochemical Immunosensor Based on Platinum Hybrid Multiwalled Carbon Nanotubes Adhered Copper Oxide

Scientific Papers | 更新时间：2023-01-04

- A Sandwich-type Electrochemical Immunosensor Based on Platinum Hybrid Multiwalled Carbon Nanotubes Adhered Copper Oxide
- Journal of Instrumental Analysis Vol. 41, Issue 11, Pages: 1664-1670(2022)
- 作者机构：
  
  1.烟台南山学院工学院，山东烟台 265713
  2.烟台南山学院化工学院，山东烟台 265713
  3.航鑫材料科技有限公司，山东烟台 264006
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.22050501
  CLC：
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孙静波,栾业波,马世花等.基于铂氧化铜负载多壁碳纳米管标记夹心型电化学免疫传感器[J].分析测试学报,2022,41(11):1664-1670.

SUN Jing-bo,LUAN Ye-bo,MA Shi-hua,et al.A Sandwich-type Electrochemical Immunosensor Based on Platinum Hybrid Multiwalled Carbon Nanotubes Adhered Copper Oxide[J].Journal of Instrumental Analysis,2022,41(11):1664-1670.
孙静波,栾业波,马世花等.基于铂氧化铜负载多壁碳纳米管标记夹心型电化学免疫传感器[J].分析测试学报,2022,41(11):1664-1670. DOI： 10.19969/j.fxcsxb.22050501.

SUN Jing-bo,LUAN Ye-bo,MA Shi-hua,et al.A Sandwich-type Electrochemical Immunosensor Based on Platinum Hybrid Multiwalled Carbon Nanotubes Adhered Copper Oxide[J].Journal of Instrumental Analysis,2022,41(11):1664-1670. DOI： 10.19969/j.fxcsxb.22050501.

摘要

设计了一种夹心型电化学免疫传感器，以金负载二氧化锡石墨烯（GS－SnO,2,－Au）为检测平台，铂氧化铜负载多壁碳纳米管（Pt@CuO－MWCNTs）为标记物，用于乙肝表面抗原（HBs）的定量检测。GS－SnO,2,－Au具有较高比表面积，良好的导电性、分散性和生物相容性，有利于捕获大量抗体（Ab,1,）。Pt、CuO和多壁碳纳米管对H,2,O,2,分解均具有催化性，Pt@CuO－MWCNTs复合物能协同放大催化作用，实现多重放大信号的效果，有利于实现高灵敏检测。通过透射电镜表征GS－SnO,2,－Au和Pt@CuO－MWCNTs的形貌特征。结果表明，基底材料和标记物材料的性能良好。在最佳实验条件下：磷酸盐pH值7.4，GS－SnO,2,－Au的质量浓度为1 mg/mL，Pt@CuO－MWCNTs质量浓度为2 mg/mL，该免疫传感器对HBs的线性范围为0.001 ~ 100 ng/mL，检出限为0.33 pg/mL，对实际样品的加标回收率为99.8% ~ 100%，相对标准偏差（RSD）为1.1% ~ 1.5%。所设计的免疫传感器具有良好选择性、重现性和稳定性，在HBs的临床检测方面具有潜在应用价值。

Abstract

A novel sandwich-type electrochemical immunosensor was designed for quantitative detection of hepatitis B surface（HBs） antigen．SnO,2, loaded graphene sheet hybridized by Au NPs（GS-SnO,2,－Au） was used as the platform．Platinum hybrid multiwalled carbon nanotubes adhered copper oxide（Pt@CuO－MWCNTs） was used as label．As the carrier material，GS－SnO,2,－Au has larger surface area，better electrical conductivity，dispersibility and biological compatibility，which is benefit for capturing antibodies（Ab,1,）．Pt，CuO and multiwalled carbon nanotubes could be used as catalysts for the decomposition of hydrogen peroxide，and Pt@CuO－MWCNTs could realize multiple amplified signals due to their synergy．The morphological characteristics of GS－SnO,2,－Au and Pt@CuO－MWCNTs were observed by transmission electron microscopy（TEM）．The results showed that the substrate material（GS－SnO,2,－Au） and the marker material（Pt@CuO－MWCNTs） have excellent properties．The synthesis of Pt@CuO－MWCNTs was further characterized by energy spectrum，and the element content was also analyzed．The assembly process of the immunosensor was characterized by cyclic voltammetry（CV） and alternating current impedance．The optimal conditions were as following：pH value of phosphate buffer solution was 7.4，concentration of GS－SnO,2,－Au was 1 mg/mL，and concentration of Pt@CuO－MWCNTs was 2 mg/mL．Under the optimal conditions，the designed immunosensor exhibited a wide linear in the range of 0.001－100 ng/mL with a low detection limit of 0.33 pg/mL for HBs．The spiked recoveries for HBs in serum samples were in the range of 99.8%－100% with relative standard deviations（RSDs） of 1.1%－1.5%．With good selectivity，reproducibility and stability，the designed immunosensor could provide potential applications in clinical monitoring of HBs.

关键词

金负载二氧化锡石墨烯铂氧化铜负载多壁碳纳米管免疫传感器乙肝表面抗原

Keywords

SnO2 loaded graphene sheet hybridized by Au NPsPt hybrid multiwalled carbon nanotubes adhered copper oxideimmunosensorhepatitis B surface antigen

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