Determination of Sialic Acid in Fish by Ultra High Performance Liquid Chromatography with DMBA Derivatization

LI Pei-pei; YAN Zhong-yong; CHEN Yin; HE Peng-fei; HUANG Li-ying; FANG Yi

doi:10.19969/j.fxcsxb.22042904

您当前的位置：

首页 >

文章列表页 >

Determination of Sialic Acid in Fish by Ultra High Performance Liquid Chromatography with DMBA Derivatization

Experimental Techniques and Methods | 更新时间：2023-01-04

- Determination of Sialic Acid in Fish by Ultra High Performance Liquid Chromatography with DMBA Derivatization
- Journal of Instrumental Analysis Vol. 41, Issue 11, Pages: 1684-1689(2022)
- 作者机构：
  
  1.浙江省海洋水产研究所，浙江舟山 316021
  2.浙江省海洋渔业资源可持续利用技术研究重点实验室，浙江舟山 316021
  3.浙江省海洋生物医用制品工程技术研究中心，浙江舟山 316021
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.22042904
  CLC：
扫描看全文
李佩佩,严忠雍,陈荫等.DMBA衍生/超高效液相色谱法测定鱼肉中唾液酸含量[J].分析测试学报,2022,41(11):1684-1689.

LI Pei-pei,YAN Zhong-yong,CHEN Yin,et al.Determination of Sialic Acid in Fish by Ultra High Performance Liquid Chromatography with DMBA Derivatization[J].Journal of Instrumental Analysis,2022,41(11):1684-1689.
李佩佩,严忠雍,陈荫等.DMBA衍生/超高效液相色谱法测定鱼肉中唾液酸含量[J].分析测试学报,2022,41(11):1684-1689. DOI： 10.19969/j.fxcsxb.22042904.

LI Pei-pei,YAN Zhong-yong,CHEN Yin,et al.Determination of Sialic Acid in Fish by Ultra High Performance Liquid Chromatography with DMBA Derivatization[J].Journal of Instrumental Analysis,2022,41(11):1684-1689. DOI： 10.19969/j.fxcsxb.22042904.

摘要

建立了同时检测鱼肉中N-乙酰神经氨酸（Neu5Ac）、N-羟乙酰神经氨酸（Neu5Gc）和酮基－脱氧壬酮糖酸（KDN）3种核心唾液酸的超高效液相色谱－荧光检测（UPLC－FLD）方法。冷冻干燥后的样品在8 mL 0.6 mol/L盐酸溶液中80 ℃酸解20 min，采用4，5-二甲基-1，2-苯二胺（DMBA）衍生试剂进行衍生。前处理后的样品经Waters Acquity UPLC BEH C,18,柱（100 mm × 2.1 mm，1.7 μm）分离，以纯水－乙腈为流动相梯度洗脱，荧光检测器检测。结果表明，Neu5Ac、Neu5Gc和KDN在0.02 ~ 5.0 μg/mL范围内呈良好的线性关系（,r,2,>, 0.999），定量下限（,S,/,N ,= 10）分别为0.20、0.20、0.10 μg/g；加标回收率为90.8% ~ 103%，相对标准偏差（RSD）为2.8% ~ 5.0%。该方法分析时间短，4 min内可完成3种唾液酸的分离，分离效果好，且方法灵敏度、准确度高，适用于鱼肉中唾液酸的测定。

Abstract

An ultra high performance liquid chromatography－fluorescence detection（UPLC－FLD） method was developed for the simultaneous determination of 3 core kinds of sialic acids（SAs） including N-acetylneuraminic acid（Neu5Ac），N-glycolylneuraminic acid（Neu5Gc） and 2-keto-3-deoxynononic acid（KDN） in fish. The freeze-dried samples were acid hydrolyzed with 8 mL 0.6 mol/L hydrochloric acid solution for 20 min at 80 ℃，followed by derivatization with 4，5-dimethylbenzene-1，2-diamine（DMBA）．The derivative conditions for DMBA were optimized by single factor test．All the target compounds were separated on an Waters Acquity UPLC BEH C,18, column（100 mm × 2.1 mm，1.7 μm） by gradient elution，using pure water and acetonitrile as eluents，then detected by UPLC－FLD．Under the optimized conditions，there were good linear relationships for Neu5Ac，Neu5Gc and KDN in the range of 0.02－5.0 μg/mL（,r,2,>, 0.999），with their limits of quantitation（,S,/,N ,= 10） of 0.20 μg/g，0.20 μg/g and 0.10 μg/g，respectively．The spiked recoveries for three SAs at three levels ranged from 90.8% to 103%，with relative standard deviations（RSDs） of 2.8%－5.0%．The analysis was rapid，and it could be achieved within 4 min with good separation and sharp peak shapes．With the characteristics of high accuracy and good precision，the method was suitable for the simultaneous determination of Neu5Ac，Neu5Gc and KDN in fish.

关键词

唾液酸超高效液相色谱衍生鱼肉

Keywords

sialic acidsultra performance liquid chromatographyderivatizationfish

references

Angata T，Varki A．Chem. Rev.，2002，102（2）：439－470.

Schauer R，Kamerling J P．Adv. Carbohyd. Chem. Biochem.，2018，75：1－213.

Varki A，Cummings R D，Esko J D．Essentials of Glycobiology. 3rd ed. Cold Spring Harbor：Cold Spring Harbor Laboratory Press，2009：784.

Yu H，Chokhawala H A，Huang S，Chen X．Nat. Protoc.，2006，1（5）：2485－2492.

Varki A，Schnaar R L，Schauer R．Sialic Acids and Other Nonulosonic Acids. 3rd ed. Cold Spring Harbor：Cold Spring Harbor Laboratory Press，2022：179－195.

Fong B Y，Ma L，Khor G L，Yvonne V D D，Rowan A，Mcjarrow P，Macgibbon A K H．J. Agric. Food Chem.，2016，64（32）：6295－6305.

Nie H，Li Y，Sun X L．J. Proteomics，2012，75（11）：3098－3112.

Varki A，Gagneux P．Ann. Ny. Acad. Sci.，2012，1253（1）：16－36.

Wang B，Brand－Miller J．Eur. J. Clin. Nutr.，2003，57（11）：1351－1369.

Rajaram S，Danasekaran B，Venkatachalapathy R，Vishnu P K，Rajaram S．Dent. Res. J. （Isfahan），2017，14（1）：267.

Goodarzi M T，Shafiei M，Nomani H，Shahriarahmadi A．Iran. J. Basic. Med. Sci.，2015，30（3）：124－127.

Krishnan K，Balasundaram S．J. Clin. Diagn. Res.，2017，11（3）：ZC25－ZC27.

Zhou X，Yang G，Guan F．Cells，2020，9（2）：273.

Cheeseman J，Kuhnle G，Daniel S I R，Osborn H M I．Bioorgan. Med. Chem.，2021，30（1）：115882.

Lacomba R，Salcedo J，Alegria A，Jesús L M，Barberá R，Matencio E．J. Pharm. Biomed.，2010，51（2）：346－357.

Feng T Y，Xue C H，Sun T，Cui H B，Xu J．Food Sci. （冯婷玉，薛长湖，孙通，崔宏博，徐杰．食品科学），2010，31（8）：233－236.

Lai Y F，Hu J，Wang Y P，Wu Z J，Zhang Q G．China Food Addit. （赖源发，胡佳，王鋆萍，吴振洁，张启国．中国食品添加剂），2015，4：185－189.

Xie H L，Li C，Liu N．Chin. J. Chromatogr. （解鸿蕾，李春，刘宁．色谱），2013，31（8）：781－785.

Ye L Y，Mu L，Li G，Yi B B．J. Food Compos. Anal.，2020，87（7）：103393.

Feng J，Yang G Y，Li H J，Han L Q，Lin M W．Food Sci. （冯君，杨国宇，李宏基，韩立强，林茂旺．食品科学），2008，29（5）：355－357.

Priego－Capotea F，Orozco－Solanoa M I，Calderón－Santiagoa M，Luque de Castroab M D．J. Chromatogr. A，2014，1346：88－96.

Yu H Z，Li Y H，Huang A S．Talanta，2021，232：122434.

Zhao F，Chen B Y，Li K F，Wang X．Food Sci. （赵非，陈宝英，李克峰，王旭．食品科学），2021，42（4）：313－318.

Ji S N，Wang F，Chen Y，Zhang P，Zhang X，Troy F A，Wang B．Glycoconjugate J.，2017，34（1）：21－30.

Wang M M．Establishment and Optimization of Method for Sialic Acid Detection from Animal-originated Foods and the High-throughput Method for Sialylated Conjunction Extraction. Nanjing：Nanjing Agricultural University（王毛毛．膳食中唾液酸测定及唾液酸复合物高通量提取方法研究），南京：南京农业大学，2015.

Samraj A N，Laubli H，Varki N，Varki A．Front. Oncol.，2014，33（4）：1－13.

Nkeonye O L，Sallau A B，Ihuoma O，Ameh D A，Okpe O，Arekemase1 S O．J. Biochem. Cell Biol.，2015，4（1）：1－9.

Yang Y，Jiao S L，Zhu M Q，Gao W Y．Chem. Eng. （杨阳，焦淑玲，朱美旗，高文运．化学工程师），2020，9：51－56.

Du J J，Zhang Q W，Li J J，Zheng Q．Anal. Methods，2020，17：2221－2227.

Zhang Q W，Zheng Q．Chin. J. Chromatogr. （张启伟，郑琦．色谱），2019，37（12）：1261－1267.

Du J J．Structural Identification of Natural Sialic Acid Isomers and the Study and Application of its Relative Quantitative Analysis Method. Wuhan：Jianghan University（杜俊杰．天然唾液酸同分异构体的结构鉴定及相对定量分析方法的研究与应用），武汉：江汉大学，2020.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Simultaneous Determination of 5 Saponins in Chonglou Xiaoliu Formula by Ultra Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection

Determination of Haloacetic Acids in Drinking Water Using Ultra Performance Liquid Chromatography Coupled with Solid phase Extraction

Simultaneous Determination of 30 Hormones and Chloramphenicols Residues in Fish Using QuEChERS Sample Preparation and Liquid Chromatography-Tandem Mass Spectrometry

Determination of Total Acetoin in Wine after Derivatization with 2-4-Dinitrophenylhydrazine by High Performance Liquid Chromatography

The Research of Reference Material Chloramphenicol in Fish

Related Author

No data

Related Institution

Guangdong Province Engineering Technology Research Institute of T..，Guangzhou

College of Pharmacy，Guangzhou Xinhua University

Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine

The Fifth Clinical Medicine School，Guangzhou University of Chinese Medicine

国家地质实验测试中心

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰