In this work，an ultrahigh performance liquid chromatography tandem time-of-flight mass spectrometry（UPLC－Q－TOF MS） with chemometric method of automatic untargeted data analysis software（AntDAS） was developed to analyze the flower samples of ,Elaeagnus angustifolia, L.．Flowers from four periods，i.e．initial bloom stage（25% of flowers），pre-full bloom stage（70%－80% of flowers），full bloom stage（100% of flowers） and the ending of the bloom stage（flowers began to wither） were collected at the location of Yinchuan，China．All samples were immediately placed into liquid nitrogen after collection，and transformed into the laboratory．Then，each flower sample was grounded to powder with a grinder．About 20 mg samples were weighted and put into a 2 mL tube，then added with 1.5 mL extraction solution（a mixture of methanol and water with volume ratio of 7∶3） to perform the metabolite extraction．A 30 min ultrasonic extraction was utilized，followed by a 15 min centrifugation at 13 000 r/min．Eventually，about 3 µL was injected into an AB SCIEX Triple TOF 5600 + MS instrument，which was connected with a Waters ACQUITY UPLC I－Class PLUS UPLC．Metabolites were separated with a chromatographic column of Waters ACQUITY UPLC BEH C,18,（2.1 mm × 100 mm，1.7 μm）．The mobile phase consisted of water and acetonitrile，both of which were added with 0.1% formic acid．The acquired data files were directly imported into AntDAS for automatically performing extracted ion chromatogram（EIC） construction，EIC peak detection，and peak registration to screen features that show significant differences among various flowering stages．At last，compound identification was performed by the AntDAS on the basis of the screened features．A total of 19 metabolites were identified based on the above-mentioned data analysis workflow，among which 12 metabolites were further confirmed by standards，including ,L-,lysine，,L-,histidine，glutamine，,L-,methionine，,L-,tyrosine，phenylalanine，,L-,tryptophan，N,-,methyl,-L-,proline，,trans,-4-coumaric acid，isorhamnetin-3-O-rutinoside，,trans,-ferulic acid and ormosanine．Results from both principal component analysis and hierarchical cluster analysis indicated that flowers of ,Elaeagnus angustifolia ,L．from different flowering stages can be clearly separated．The heatmap analysis suggested that compounds from various flowering stages show significantly different expression levels．For example，majority of amino acids（,L-,tryptophan，,L-,lysine，,L-,histidine，,L-,tyrosine and ,L-,methionine） were expressed at higher levels during the full bloom and the ending of the bloom stages when compared with the initial bloom and the pre-full bloom stages．The study may be helpful for the quality evaluation of flowers of ,Elaeagnus angustifolia ,L.．Additionally，the developed strategy provides a new solution for current plant-related research works.