Determination of 12 Saccharides in Honey by Ultra High Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection

JIA Ru; HUANG Xue-zhe; JIA Guang-qun; SHI Yu-qiu; CUI Zong-yan; GUO Ze-bin

doi:10.19969/j.fxcsxb.21101901

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Determination of 12 Saccharides in Honey by Ultra High Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection

Scientific Papers | 更新时间：2022-06-22

- Determination of 12 Saccharides in Honey by Ultra High Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection
- Journal of Instrumental Analysis Vol. 41, Issue 6, Pages: 851-857(2022)
- 作者机构：
  
  1.福建农林大学食品科学学院，福建福州 350002
  2.秦皇岛海关技术中心，河北秦皇岛 066004
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.21101901
  CLC：
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贾茹,黄学者,贾光群等.超高效液相色谱－蒸发光散射检测法测定蜂蜜中12种糖组分[J].分析测试学报,2022,41(06):851-857.

JIA Ru,HUANG Xue-zhe,JIA Guang-qun,et al.Determination of 12 Saccharides in Honey by Ultra High Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection[J].Journal of Instrumental Analysis,2022,41(06):851-857.
贾茹,黄学者,贾光群等.超高效液相色谱－蒸发光散射检测法测定蜂蜜中12种糖组分[J].分析测试学报,2022,41(06):851-857. DOI： 10.19969/j.fxcsxb.21101901.

JIA Ru,HUANG Xue-zhe,JIA Guang-qun,et al.Determination of 12 Saccharides in Honey by Ultra High Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection[J].Journal of Instrumental Analysis,2022,41(06):851-857. DOI： 10.19969/j.fxcsxb.21101901.

摘要

建立了蜂蜜中果糖、葡萄糖、蔗糖、松二糖、麦芽酮糖、麦芽糖、曲二糖、异麦芽糖、吡喃葡糖基蔗糖、松三糖、蜜三糖和麦芽三糖含量的超高效液相色谱－蒸发光散射测定方法。试样经去离子水溶解，乙腈定容，用0.22 μm滤膜过滤后，采用Amide色谱柱分离，蒸发光散射检测器检测，外标法定量。在优化实验条件下，12种糖得到良好分离，线性相关系数为0.997 7～0.999 8，果糖和葡萄糖的检测结果与示差折光检测法无显著性差异，其余10种糖的平均回收率为90.0 %～105 %，相对标准偏差为0.70%～5.8%。该方法灵敏度高，准确度高，重复性好，适用于蜂蜜中多种糖组分的同时定量分析。用于72批不同蜜种蜂蜜样品中糖组分的检测，发现中国主要蜜源蜂蜜中普遍含有松二糖、麦芽酮糖、麦芽糖等糖组分。该方法和检测数据可为蜂蜜品质和质量评价提供重要支撑。

Abstract

An ultra performance liquid chromatography（UPLC） coupled with evaporative light scattering detection（ELSD） was established for the determination of 12 saccharides，i.e．fructose，glucose，sucrose，turanose，mlatulose，maltose，kojibiose，isomaltose，erlose，melezitose，raffinose and maltotriose in honey. Honey sample was dissolved in deionized water and kept constant volume with acetonitrile．The solution was then filtered with a 0.22 μm filter membrane．12 saccharides were separated on an Amide column，then determined with an evaporative light scattering detector，and finally quantified by external standard calibration method．Through the optimization experiment，there existed good linear relationships for 12 saccharides，and the linear correlation coefficients were in the range of 0.997 7－0.999 8．The obtained results for fructose and glucose by this method were comparable to those of the traditional refractive index detection method．The average recoveries for the other 10 saccharides ranged from 90.0% to 105%，and the relative standard deviations were between 0.70% and 5.8%．The method was validated to be sensitive，accurate and repeatable．It was suitable for simultaneous quantitative analysis of multiple sugar components in honey．The proposed method was applied to the determination of 12 saccharides in 72 batches of honey samples of different botanical origins．It was found that turanose，mlatulose and maltose are the dominant disaccharides in Chinese honeys．This method and test data provided an important support for honey quality and quality evaluation．

关键词

蜂蜜糖分高效液相色谱蒸发光散射

Keywords

honeysugarhigh performance liquid chromatographyevaporative light scattering detection

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