LI Yang,SHAN Yi-chu,LIANG Zhen,et al.Research Advances of Enrichment Approaches for SUMOylated Proteome[J].Journal of Instrumental Analysis,2022,41(01):58-62.
LI Yang,SHAN Yi-chu,LIANG Zhen,et al.Research Advances of Enrichment Approaches for SUMOylated Proteome[J].Journal of Instrumental Analysis,2022,41(01):58-62. DOI: 10.19969/j.fxcsxb.21092402.
Research Advances of Enrichment Approaches for SUMOylated Proteome
Protein SUMOylation is one of the most important post-translational modifications,which involved in various biological processes,including nuclear transport,transcriptional regulation,maintenance of gene integrity and cell cycle.However,identification of SUMOylation has always been the hotspot and difficulty in this field due to the low abundance and lengthy peptide sequence of endogenous SUMOylation.Therefore,the efficient enrichment of SUMOylated proteins/peptides is necessary to achieve deep coverage analysis of SUMOylome.The basic principles,characteristics and latest progresses of different enrichment methods for SUMOylated proteome are reviewed,and the development prospects are prospected.
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