Detection of <italic style="font-style: italic">Vibrio Parahaemolyticus </italic>by an Aptameric Fluorescence Method Based on Metal Organic Framework Material and Enzyme-assisted Signal Amplification

FENG Run-chan; YUAN Ping; TAN Shu-liang; WU Fu-hai; SU Zheng-quan; BAI Yan; HE Jin-can

doi:10.19969/j.fxcsxb.21072401

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Detection of Vibrio Parahaemolyticus by an Aptameric Fluorescence Method Based on Metal Organic Framework Material and Enzyme-assisted Signal Amplification

Scientific Papers | 更新时间：2022-07-01

- Detection of Vibrio Parahaemolyticus by an Aptameric Fluorescence Method Based on Metal Organic Framework Material and Enzyme-assisted Signal Amplification
- Journal of Instrumental Analysis Vol. 41, Issue 3, Pages: 348-353(2022)
- 作者机构：
  
  广东药科大学公共卫生学院，广东广州 510310
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.21072401
  CLC：
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冯润婵,袁萍,谭树良等.基于金属有机骨架材料和酶切放大的适配体荧光法检测副溶血性弧菌[J].分析测试学报,2022,41(03):348-353.

FENG Run-chan,YUAN Ping,TAN Shu-liang,et al.Detection of Vibrio Parahaemolyticus by an Aptameric Fluorescence Method Based on Metal Organic Framework Material and Enzyme-assisted Signal Amplification[J].Journal of Instrumental Analysis,2022,41(03):348-353.
冯润婵,袁萍,谭树良等.基于金属有机骨架材料和酶切放大的适配体荧光法检测副溶血性弧菌[J].分析测试学报,2022,41(03):348-353. DOI： 10.19969/j.fxcsxb.21072401.

FENG Run-chan,YUAN Ping,TAN Shu-liang,et al.Detection of Vibrio Parahaemolyticus by an Aptameric Fluorescence Method Based on Metal Organic Framework Material and Enzyme-assisted Signal Amplification[J].Journal of Instrumental Analysis,2022,41(03):348-353. DOI： 10.19969/j.fxcsxb.21072401.

摘要

基于铜基金属有机骨架（Cu－MOF）的荧光猝灭性质、核酸适配体的选择识别能力及核酸外切酶的水解放大信号作用，建立了一种快速检测副溶血性弧菌（V.P）的适配体荧光法。该文在室温下合成Cu－MOF，对其形貌、结构、荧光猝灭机理进行了系统研究，考察了Cu－MOF的体积、猝灭时间、核酸外切酶用量、恢复时间及试剂加入顺序等分析条件的影响。当V.P的浓度（,c,）在8.44 × 10,2,～2.03 × 10,5, CFU/mL范围内时，浓度对数值（lg,c,）与荧光信号恢复程度（,∆F,）存在一定的线性关系，线性方程为,∆F, = 7.30lg,c,－19.58（,r,2, = 0.998 6），检出限为37 CFU/mL，整个分析过程不超过30 min。将该方法应用于虾、八爪鱼样品中V.P的检测，加标回收率为88.8%～109%。该方法快速、灵敏、选择性好，对复杂样品中V.P的分析具有一定的应用潜力。

Abstract

Based on the fluorescence quenching property of copper-based metal organic framework（Cu－MOF），the selective recognition ability of aptamers and the catalytic hydrolysis of exonuclease，a rapid aptameric fluorescence method was developed for the detection of ,Vibrio parahaemolyticus,（V.P）．The Cu－MOF was prepared at room temperature．The morphology，structure and fluorescence quenching mechanism of Cu－MOF were systematically studied．The analytical conditions such as volume of Cu－MOF，quenching time，volume of exonuclease，recovery time and addition order of agents were investigated．Based on the optimized conditions，an aptameric fluorescence method was established for the detection of V.P．There was a linear relationship between logarithm of concentration（lg,c,） and increase of fluorescence recovery intensity（,∆F,） in the range of 8.44 × 10,2,－2.03 × 10,5, CFU/mL，with a correlation coefficient（,r,2,） of 0.998 6 and a linear equation of ,∆F, = 7.30lg,c,－19.58．The detection of limit was 37 CFU/mL．The method could be carried out within 30 min．The proposed method was applied to the determination of V.P in shrimp and octopus samples with recoveries of 88.8%－109%．The method is rapid，sensitive and selective，and has a certain application potential in the analysis of V.P in complex samples.

关键词

副溶血性弧菌（V.P）核酸适配体金属有机骨架核酸外切酶荧光分析

Keywords

Vibrio Parahaemolyticus（V.P）aptamermetal organic frameworksexonucleasefluorescence method

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College of Biological and Agricultural Science and Technology，Zunyi Normal University

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Detection of Vibrio Parahaemolyticus by an Aptameric Fluorescence Method Based on Metal Organic Framework Material and Enzyme-assisted Signal Amplification

DOI：10.19969/j.fxcsxb.21072401

摘要

Abstract

关键词

Keywords

references