Screening of potential risk factors in food is very important for the ensurement of food safety．Pesticide residue is one of the important factors affecting the quality and safety of agricultural products，and the multi-residue detection of pesticides in complex matrices is still a challenging task．A rapid non-targeted screening strategy for pesticide residues in fruits and vegetables was proposed in this study，based on ultra-performance liquid chromatography－high resolution mass spectrometry（UHPLC－HRMS）．Meanwhile，an analytical method for pesticide residues in complex substrates was established，including rapid sample pretreatment，accurate separation by UHPLC and detection by HRMS．Brief steps for the method construction are described as follows．Firstly，the pre-treatment method including purification agents and extraction solvents were optimized，and the acetonitrile containing 1% acetate and dispersive solid phase extraction with 20 mg PSA + 20 mg C,18, + 2.5 mg GCB + 150 mg MgSO,4, were selected for sample preparation，owing to its relatively high recoveries for most of pesticides．Secondly，the instrument conditions were optimized，the separation was performed on an Accucore aQ C,18, column，and the high-throughput qualitative screening and quantitative detection were performed by Full Scan/ dd－MS,2,．More importantly，the retention time correction strategy was introduced to calibrate the retention time generated under different LC－MS conditions．Finally，a high-resolution database containing 651 pesticides was established based on the original commercial database and the actual situation of the limits of different pesticides on agricultural products in various countries．Under the optimized conditions，108 kind of pesticides in fruit and vegetable matrix were analyzed．Results showed that there were good linear relationships for all the pesticides in the concentration range of 5－500 μg/L，with their correlation coefficients（,r,2,） larger than 0.99．The limits of detection（LODs，,S,/,N, ≥ 3） for all the pesticides were in the range of 0.002－1.5 μg/kg，while the limits of quantitation（LOQs） were 5 μg/kg．At three spiked levels of 5，25，100 μg/kg，the recoveries for 106 pesticides except cyromazine and chlormequat chloride ranged from 61.2% to 120%，with relative standard deviations（RSD） of 0.1%－9.9%．The established method was successfully applied to the analysis of fifty－eight cowpea and twelve citrus samples for pesticides screening．Results showed that this method was rapid，accurate and sensitive，and was suitable for the rapid screening and quantitative analysis of unknown pesticide residues in agricultural products.