Label⁃free and Sensitive Detection of Biothiols Using a Turn⁃on Fluorescent Nanoprobe Based on Protein⁃templated Gold Nanoclusters Coupled with Cobalt Oxyhydroxide Nanoflakes

LI Shuo; ZHANG Gui⁃hua; WANG Yu⁃min; YU Ying; LIN Bi⁃xia; ZHANG Li; CAO Yu⁃juan; GUO Man⁃li

doi:10.19969/j.fxcsxb.21032904

您当前的位置：

首页 >

文章列表页 >

Label⁃free and Sensitive Detection of Biothiols Using a Turn⁃on Fluorescent Nanoprobe Based on Protein⁃templated Gold Nanoclusters Coupled with Cobalt Oxyhydroxide Nanoflakes

Scientific Papers | 更新时间：2023-02-13

- Label⁃free and Sensitive Detection of Biothiols Using a Turn⁃on Fluorescent Nanoprobe Based on Protein⁃templated Gold Nanoclusters Coupled with Cobalt Oxyhydroxide Nanoflakes
- Journal of Instrumental Analysis Vol. 40, Issue 12, Pages: 1751-1757(2021)
- 作者机构：
  
  华南师范大学化学学院广州市生物医药分析化学重点实验室，广东广州 510006
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.21032904
  CLC：
扫描看全文
李硕,张桂华,王郁敏等.基于蛋白模板金纳米簇及羟基氧化钴纳米片的激活型荧光纳米探针用于免标记和灵敏检测生物硫醇[J].分析测试学报,2021,40(12):1751-1757.

LI Shuo,ZHANG Gui⁃hua,WANG Yu⁃min,et al.Label⁃free and Sensitive Detection of Biothiols Using a Turn⁃on Fluorescent Nanoprobe Based on Protein⁃templated Gold Nanoclusters Coupled with Cobalt Oxyhydroxide Nanoflakes[J].Journal of Instrumental Analysis,2021,40(12):1751-1757.
李硕,张桂华,王郁敏等.基于蛋白模板金纳米簇及羟基氧化钴纳米片的激活型荧光纳米探针用于免标记和灵敏检测生物硫醇[J].分析测试学报,2021,40(12):1751-1757. DOI： 10.19969/j.fxcsxb.21032904.

LI Shuo,ZHANG Gui⁃hua,WANG Yu⁃min,et al.Label⁃free and Sensitive Detection of Biothiols Using a Turn⁃on Fluorescent Nanoprobe Based on Protein⁃templated Gold Nanoclusters Coupled with Cobalt Oxyhydroxide Nanoflakes[J].Journal of Instrumental Analysis,2021,40(12):1751-1757. DOI： 10.19969/j.fxcsxb.21032904.

摘要

该文基于牛血清白蛋白模板金纳米簇（BSA@AuNCs）与羟基氧化钴（CoOOH）纳米片构建了一种激活型荧光纳米探针用于生物硫醇的检测。带负电的BSA@AuNCs能通过静电吸附作用组装到带正电的CoOOH纳米片表面，与此同时，BSA@AuNCs的荧光由于内滤效应（IFE）有效地被CoOOH纳米片猝灭，形成BSA@AuNCs－CoOOH纳米探针。当向纳米探针溶液加入生物硫醇（0.05～150 μmol/L）时，生物硫醇与纳米探针中的CoOOH纳米片发生氧化还原反应，CoOOH纳米片被降解生成Co,2+,，同时释放出BSA@AuNCs，BSA@AuNCs荧光信号恢复。结果表明，该纳米探针可以检测低浓度的生物硫醇，对生物硫醇（半胱氨酸、谷胱甘肽和高半胱氨酸）的检出限为30 nmol/L。相对于其他的氨基酸、金属离子及糖类化合物，该纳米探针对生物硫醇具有较高的选择性并成功应用于人血清样品中生物硫醇的检测。

Abstract

Cobalt oxyhydroxide（CoOOH）nanoflakes，an emerging and promising functional two⁃dimensional（2D）nanomaterial with remarkable physical and chemical properties，have received much attention in recent years.Until now，all kinds of fluorescent nanoprobes by integrating CoOOH nanoflakes with fluorescent inorganic nanomaterials have been reported.However，the synthesis procedures for fluorescent inorganic nanomaterials employed in these nanoprobes are generally time⁃consuming and complex，and most of them are limited to sensing ascorbic acid. Therefore，investigation on the interaction between CoOOH nanoflakes and biomolecule，and the development of new CoOOH⁃based fluorescent nanoprobe to broaden biological applications is very interesting and also of great importance.Herein，a turn⁃on fluorescent nanoprobe based on CoOOH nanoflakes and BSA⁃templated gold nanoclusters（BSA@AuNCs）was constructed for sensitive and label⁃free detection of biothiols.It was found that negative BSA@AuNCs were easily and effectively assemblied on the surface of positive CoOOH nanoflakes to construct a BSA@AuNCs－CoOOH nanoprobe in a short time through the electrostatic interaction between scaffold protein BSA and CoOOH nanoflakes.As a result，the red strong fluorescence emitted by BSA@AuNCs could be efficiently quenched by CoOOH nanoflakes through inner⁃filter effect（IFE）.The CoOOH nanoflakes in the BSA@AuNCs－CoOOH nanoprobe were degraded in the presence of the target biothiol due to the specific redox reaction between biothiols and CoOOH nanoflakes，leading to the release and fluorescence recovery of BSA@AuNCs.On the basis of the emission increase of BSA@AuNCs，a robust fluorescence sensing strategy for sensitive and selective detection of biothiols was developed，which was demonstrated to be suitable for the quantitative analysis of biothiols（Cys，GSH and Hcy）in the range of 0.‍05－150 μmol/L，with a detection limit as low as 30 nmol/L.Furthermore，this nanoprobe exhibited a high selectivity towards biothiols other than amino acids，metal ions and saccharides.The practical application of this nanoprobe was verified，and it was successfully applied to the detection of biothiol（Cys）in human serum samples.The developed biosensing strategy might provide a simple，facile，cost⁃efficient and rapid platform for biothiols⁃related clinical disease diagnosis.

关键词

荧光纳米探针CoOOH纳米片蛋白与CoOOH相互作用蛋白模板金纳米簇免标记生物硫醇

Keywords

fluorescent nanoprobeCoOOH nanoflakesprotein－CoOOH interactionprotein⁃templated gold nanoclusterslabel⁃freebiothiols

references

Chen Y，Fan Z X，Zhang Z C，Niu W X，Li C L，Yang N L，Chen B，Zhang H．Chem. Rev.，2018，118（13）：6409－6455.

Su S，Sun Q，Gu X D，Xu Y Q，Shen J L，Zhu D，Chao J，Fan C H，Wang L H．Trends Anal. Chem.，2019，119：115610.

Wu J H，Lu Y H，Feng S R，Wu Z Q，Lin S Y，Hao Z Z，Yao T Y，Li X M，Zhu H W，Lin S S．Adv. Funct. Mater.，2018，28（50）：1804712.

Tuček J，Błoński P，Ugolotti J，Swain A K，Enoki T，Zbořil R．Chem. Soc. Rev.，2018，47（11）：3899－3990.

Ju Y，Hu X，Zang Y，Cao R，Xue H G．Anal. Methods，2019，11（16）：2163－2169.

Liu H，Yin H，Dong Y P，Ding H C，Chu X F．Analyst，2020，145（6）：2204－2211.

Rasheed P A，Pandey R P，Jabbar K A，Ponraj J，Mahmoud K A．Anal. Methods，2019，11（30）：3851－3856.

Huang S Z，Kang T F，Lu L P．J. Instrum. Anal. （黄素珍，康天放，鲁理平.分析测试学报），2016，35（1）：48－53.

Zhu X H，Fan L，Wang S G，Lei C Y，Huang Y，Nie Z，Yao S Z．Anal. Chem.，2018，90（11）：6742－6748.

Liu Y M，Sun Y N，Yang M L．Anal. Methods，2021，13（7）：903－909.

Cheng L，Liu J J，Gu X，Gong H，Shi X Z，Liu T，Wang C，Wang X Y，Liu G，Xing H Y，Bu W B，Sun B Q，Liu Z．Adv. Mater.，2014，26（12）：1886－1893.

Chai D D，Hao B J，Hu R，Zhang F，Yan J，Sun Y，Huang X Y，Zhang Q X，Jiang H．ACS Appl. Mater. Interfaces，2019，11（26）：22915－22924.

Chng E L K，Sofer Z，Pumera M．Nanoscale，2014，6（23）：14412－14418.

Qu G B， Liu W，Zhao Y T，Gao J，Xia T，Shi J B，Hu L G，Zhou W H，Gao J J，Wang H Y，Luo Q，Zhou Q F，Liu S J，Yu X F，Jiang G B．Angew. Chem. Int. Ed.，2017，56（46）：14488－14493.

Fester J，Makoveev A，Grumelli D，Gutzler R，Sun Z Z，Rodríguez－Fernández J，Kern K，Lauritsen J V．Angew. Chem. Int. Ed.，2018，57（37）：11893－11897.

Sheng Y H，Cao T W，Xiao Y，Zhang X H，Wang S F，Liu Z H．Analyst，2019，144（11）：3511－3517.

Feng L L，Wu Y X，Zhang D L，Hu X X，Zhang J，Wang P，Song Z L，Zhang X B，Tan W．Anal. Chem.，2017，89（7）：4077－4084.

Li G L，Kong W H，Zhao M，Lu S M，Gong P W，Chen G，Xia L，Wang H，You J M，Wu Y N．Biosens. Bioelectron.，2016，79：728－735.

Lu Q J，Chen X G，Liu D，Wu C Y，Liu M L，Li H T，Zhang Y Y，Yao S Z．Microchim. Acta，2019，186（2）：72.

Wu Z H，Nan D Y，Yang H，Pan S，Liu H，Hu X L．Anal. Chim. Acta，2019，1091：59－68.

Chang Y Q，Zhang Z，Liu H Q，Wang N，Tang J L．Analyst，2016，141（15）：4719－4724.

Cen Y，Yang Y，Yu R Q，Chen T T，Chu X．Nanoscale，2016，8（15）：8202－8209.

Wu Y Z，Pramanik G，Eisele K，Weil T J．Biomacromolecules，2012，13（6）：1890－1898.

Yang W T，Guo W S，Chang J，Zhang B B．J. Mater. Chem. B，2017，5（3）：401－417.

Hua Z D，Cheng Z Y，Li Y Z，Zhao M P．Langmuir，2008，24（11）：5773－5780.

Chen S，Wang J，Hou P，Liu L，Wang X．J. Instrum. Anal. （陈颂，王静，侯鹏，刘磊，王鑫.分析测试学报），2016，35（8）：1046－1049.

Xiong Y H，Chen S H，Ye F G，Su L J，Zhang C，Shen S F，Zhao S L．Chem. Commun.，2015，51（22）：4635－4638.

Zhao X X，Lin X，Wang J H，Chen X W．New J. Chem.，2019，43（6）：2790－2796.

Ros－Lis J V，García G，Jiménez D G，Martínez－Máñez R，Sancenón F，Soto J，Gonzalvo F，Valldecabres M C．J. Am. Chem. Soc.，2004，126（13）：4064－4065.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Research Progress of Design，Synthesis and Application of Fluorescent Nanoprobe

Recent Advances in Metal Nanoclusters-based Fluorescent Platforms for Biosensing

Synthesis of a Fluorescent Probe and Its Application in Thiols Determination

Related Author

No data

Related Institution

Institute of Medical Support Technology，Academy of System Engineering of Academy of Military Sciences

吉林大学化学学院分析化学系

齐齐哈尔医学院药学院

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰