Detection of 17<italic style="font-style: italic">β</italic>-Estradiol in Water by Time-resolved Fluorescence Lateral Flow Assay

WANG Ling-ling; WANG Wen-long; YANG Cheng; XU Zheng-hua; ZHANG Yi; SHI Xue-li

doi:10.19969/j.fxcsxb.21010802

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Detection of 17β-Estradiol in Water by Time-resolved Fluorescence Lateral Flow Assay

Scientific Papers | 更新时间：2023-02-13

- Detection of 17β-Estradiol in Water by Time-resolved Fluorescence Lateral Flow Assay
- Journal of Instrumental Analysis Vol. 40, Issue 10, Pages: 1409-1416(2021)
- 作者机构：
  
  1.江南大学食品科学与技术国家重点实验室，江苏无锡 214122
  2.江南大学食品学院　分析食品安全学研究所，江苏无锡 214122
  3.中华人民共和国黄埔海关，广东广州 510730
  4.石家庄市妇幼保健院，河北石家庄 050051
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.21010802
  CLC：
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王凌凌,王文龙,杨成等.时间分辨荧光侧流分析法检测水中17β-雌二醇[J].分析测试学报,2021,40(10):1409-1416.

WANG Ling-ling,WANG Wen-long,YANG Cheng,et al.Detection of 17β-Estradiol in Water by Time-resolved Fluorescence Lateral Flow Assay[J].Journal of Instrumental Analysis,2021,40(10):1409-1416.
王凌凌,王文龙,杨成等.时间分辨荧光侧流分析法检测水中17β-雌二醇[J].分析测试学报,2021,40(10):1409-1416. DOI： 10.19969/j.fxcsxb.21010802.

WANG Ling-ling,WANG Wen-long,YANG Cheng,et al.Detection of 17β-Estradiol in Water by Time-resolved Fluorescence Lateral Flow Assay[J].Journal of Instrumental Analysis,2021,40(10):1409-1416. DOI： 10.19969/j.fxcsxb.21010802.

摘要

以偶联17,β,-雌二醇包被原（E2－BSA）的长余辉粒子（PLPs）为荧光供体，胶体金标记抗体为荧光受体，基于免疫识别和荧光共振能量转移原理，建立了E2的竞争型时间分辨荧光侧流分析方法。创新性地以余辉优良、微米级大尺寸的PLPs为荧光信号源，并利用其在硝酸纤维素膜上不迁移的特性将E2竞争物固定于检测区，巧妙解决了常规试纸对信号材料尺寸的纳米级别限制和小尺寸PLPs余辉短暂的矛盾；采用低功率紫外灯为激发光源，以智能手机实现时间分辨荧光（TRF）模式荧光采集，有效去除试纸荧光背景干扰进而提高信噪比。该时间分辨荧光侧流分析（TRF－LFA）法对E2的检出限为0.1 ng/mL，比胶体金侧流分析（CG－LFA）降低了2个数量级，且特异性良好，30 min可完成检测。将该方法应用于水中E2的检测，与HPLC－FLD法的结果一致。

Abstract

A competitive time-resolved fluorescence（TRF） lateral flow assay for 17,β,-estradiol（E2） was established with 17,β,-estradiol－BSA（E2－BSA） coupled persistent luminescence particles（PLPs） as fluorescence donor and colloidal gold（CG）-labelled antibody as fluorescence receptor，based on immune recognition and fluorescence resonance energy transfer principle．In this method，the PLPs with long persistent luminescence but large size of micron were applied as the fluorescence signal source，and its non-migration property on nitrocellulose membrane was used to fix the E2 competitor in the test zone，thus the contradiction between the limitation that the signal material of traditional test paper must be nanometer size and the short luminous lifetime of small PLPs was skillfully solved．In addition，a low-power UV lamp was used as the light source to excite the PLPs，and the continuous shooting function of smart phone was used to realize TRF－mode fluorescence acquisition，effectively removing the fluorescent background interference from the test strips and improving the signal-to-noise ratio．The detection limit of this method was 0.1 ng/mL，which was about two orders of magnitude more sensitive than those of colloidal gold and HPLC－FLD method．The detection limit of this method was 0.1 ng/mL，which was about two orders of magnitude more sensitive than that of colloidal gold．The method showed good selectivity and could perform detection in 30 min．Using this method to detect E2 in a water sample and the results were consistent with that of HPLC-FLD method.

关键词

时间分辨荧光侧流分析（LFA）17β-雌二醇荧光共振能量转移长余辉粒子

Keywords

time-resolved fluorescencelateral flow assay（LFA）17β-estradiolfluorescence resonance energy transferpersistent luminescence particle

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Detection of 17β-Estradiol in Water by Time-resolved Fluorescence Lateral Flow Assay

DOI：10.19969/j.fxcsxb.21010802

摘要

Abstract

关键词

Keywords

references