Quantitative Determination of Lactulose in Liquid Milk by High Performance Liquid Chromatography with Evaporative Light Scattering Detection

SUN Pei; REN Shuo; CHEN Rou-han; GU Shu-qing; NIU Bing; DENG Xiao-jun

doi:10.19969/j.fxcsxb.21010101

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Quantitative Determination of Lactulose in Liquid Milk by High Performance Liquid Chromatography with Evaporative Light Scattering Detection

Experimental Techniques and Methods | 更新时间：2023-02-13

- Quantitative Determination of Lactulose in Liquid Milk by High Performance Liquid Chromatography with Evaporative Light Scattering Detection
- Journal of Instrumental Analysis Vol. 40, Issue 9, Pages: 1391-1395(2021)
- 作者机构：
  
  1.上海大学生命科学学院食品工程系，上海 200436
  2.上海海关动植物与食品检验检疫技术中心，上海 200135
  3.上海市农产品质量安全中心，上海 201708
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.21010101
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孙佩,任硕,陈柔含等.高效液相色谱－蒸发光散射法测定液态乳中乳果糖含量[J].分析测试学报,2021,40(09):1391-1395.

SUN Pei,REN Shuo,CHEN Rou-han,et al.Quantitative Determination of Lactulose in Liquid Milk by High Performance Liquid Chromatography with Evaporative Light Scattering Detection[J].Journal of Instrumental Analysis,2021,40(09):1391-1395.
孙佩,任硕,陈柔含等.高效液相色谱－蒸发光散射法测定液态乳中乳果糖含量[J].分析测试学报,2021,40(09):1391-1395. DOI： 10.19969/j.fxcsxb.21010101.

SUN Pei,REN Shuo,CHEN Rou-han,et al.Quantitative Determination of Lactulose in Liquid Milk by High Performance Liquid Chromatography with Evaporative Light Scattering Detection[J].Journal of Instrumental Analysis,2021,40(09):1391-1395. DOI： 10.19969/j.fxcsxb.21010101.

摘要

液态乳中乳糖和乳果糖是同分异构体，因保留特性相似，成为色谱法分离检测乳果糖的难点。目前，虽有多种液相色谱检测乳果糖的报道，但方法在前处理的简便性、色谱柱的适用性及分离效果的稳定性等方面存在不足。该研究建立了高效液相色谱－蒸发光散射（HPLC－ELSD）检测液态乳中乳果糖的方法。采用0.4 mol/L醋酸铵（pH 3.8）沉降蛋白，氨基色谱柱分离，蒸发光散射检测器检测。液态乳中乳果糖与乳糖得到了良好的分离，在1.0～100 mg/L范围内线性关系良好（,r,2, = 0.998 0），检出限为15 mg/L，定量下限为50 mg/L，回收率为85.1%～110%，相对标准偏差（RSD）不大于7.5%。该方法操作简单，可在8 min内完成检测，结果重复性好、准确度高，满足液态乳中乳果糖的日常检测要求。

Abstract

Lactose and lactulose in liquid milk are isomers with similar retention properties， which makes separation and detection on lactulose by chromatography difficult. At present， although a variety of liquid chromatography detection methods have been reported， there are some problems in the simplicity of the pretreatment， the applicability of the chromatographic column and the stability of the separation effect. A method for the determination of lactulose in liquid milk by high performance liquid chromatography with evaporative light scattering detection（HPLC－ELSD）was developed. The protein was deposited with 0.4 mol/L ammonium acetate（pH 3.8）. The sugar analysis column was used for separation， and the evaporative light scattering detector was applied to detection. There was a good linear relationship for lactulose in liquid milk in the range of 1.0－100 mg/L， with a correlation coefficient（,r,2,） of 0.998 0. The detection limit and the quantitative limit were 15 mg/L and 50 mg/L， respectively. The recoveries were in the range of 85.1%－110%， with relative standard deviations（RSDs） not more than 7.5%. The method is simple to operate， and could be completed within 8 min. It could meet the requirements for daily detection of lactulose with good repeatability and high accuracy.

关键词

乳果糖高效液相色谱蒸发光散射液态乳

Keywords

lactulosehigh performance liquid chromatographyevaporative light scatteringliquid milk

references

Neves L N D， Marques R， da Silva P H F， de Oliveira M A L. Food Chem.， 2018， 258（30）： 337－342.

Ritota M， Di Costanzo M G， Mattera M， Manzi P. J. Anal. Methods Chem.， 2017， 2017： 1864832.

Cataldi T R I， Angelotti M， Bufo S A. Anal. Chem.， 1999， 71（21）： 4919－4925.

Morales F J， Romero C， Jiménez－Pérez S. Int. J. Food Sci. Technol.， 2000， 35（2）： 193－200.

ISO 11285－2004. Milk － Determination of Lactulose Content － Enzymatic Method. Standards of the International Organization for Standardization（牛奶. 乳糖含量的测定. 酶催化方法. 国际标准化组织标准）.

NY/T 939－2016. Identification of Reconstituted Milk in Pasteurized and UHT Milk. Agricultural Industry Standards of the People's Republic of China（巴氏杀菌乳和UHT灭菌乳中复原乳的鉴定. 中华人民共和国农业行业标准）.

Liu Y， Yue H， Zhao Z， Liu L J， Li C Z， Shao J B. Dairy Sci. Technol.（刘洋，岳虹，赵贞，刘丽君，李翠枝，邵建波. 乳业科学与技术）， 2017， 40（1）： 19－22.

Yu B H， Du Y P， Xie X B， Luan S R. Chin. J. Anal. Lab.（于博昊，杜一平，谢小波，栾绍嵘. 分析试验室）， 2010， 29（3）： 119－121.

Generoso M， De Rosa M， De Rosa M， De Magistris L， Secondulfo M， Fiandra R， Carratù R， Cartenì M. J. Chromatogr. B， 2003， 783（2）： 349－357.

Nagaraj V. Technological Approaches for Novel Applications in Dairy Processing. London: InTechOpen， 2018: 128－147.

Montilla A， Moreno F J， Olano A. Chromatographia， 2005， 62（5/6）： 311－314.

Manzi P， Pizzoferrato L. Food Bioprocess Technol.， 2013， 6（3）： 851－857.

ISO 11868—2007. Heat－Treated Milk－Determination of Lactulose－Method Using High－performance Liquid Chromatography， Standards of the International Organization for Standardizaiton（热处理牛奶．乳果糖含量的测定. 高效液相色谱法. 国际标准化组织标准）.

Jungbauer A， Hahn R. Methods Enzymol.， 2009， 463： 349－371.

Mu W M， Li Q X， Fan C， Zhou C， Jiang B. Appl. Microbiol. Biotechnol.， 2013， 97（5）： 1821－1827.

Elliott A J， Datta N， Amenu B， Deeth H C. J. Dairy Res.， 2005， 72（4）： 442－446.

Liu H T， Huang R M， Zeng G F， Xu Z L， Sun Y M， Lei H T， Sheng Y D， Wang H M， Xu B J， Wei X Q. LWT－Food Sci. Technol.， 2010， 117： 108648.

Liu M M， Song G. China Dairy Ind.（刘萌萌，宋戈. 中国乳品工业）， 2018， 46（3）： 49－51.

Schuster－Wolff－Bühring R， Michel R， Hinrichs J. Dairy Sci. Technol.， 2011， 91（1）： 27－37.

Schmidt C M， Zürn T， Thienel K J F， Hinrichs J. Food Chem.， 2017， 215（15）： 347－353.

Li S N， Zhang M J， Hu H X， Feng P Y， Zeng J， Wei X Q. Sci. Technol. Food Ind.（李盛楠，张敏婕，胡浩鑫，冯沛彦，曾静，韦晓群. 食品工业科技）， 2018， 4（10）： 998－1003.

Wang Q E， Ding M Y. J. Instrum. Anal.（王巧娥，丁明玉. 分析测试学报）， 2006， 25（6）： 126－132.

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