LIU Yu-hong,GUO Bin,TU Yi-feng.Study on Adsorptive Voltammetric Detection of Coenzyme Q10[J].Journal of Instrumental Analysis,2021,40(08):1224-1229. DOI: 10.19969/j.fxcsxb.20121806.
Study on Adsorptive Voltammetric Detection of Coenzyme Q10
The electrochemical behavior of coenzyme Q10(CoQ10) on glassy carbon electrode(GCE) was explored by cyclic voltammetry(CV) in this paper. The results revealed the adsorption of CoQ10 on GCE, thus providing an approach to enhance the sensitivity for its detection by pre-enrichment. The experimental conditions including ultrasonic duration for CoQ10 extraction, detection temperature and the ratio of electrolyte solvent (methanol-ethanol) were optimized by Box-Behnken method as 1 min 50 s, 25 ℃ and 1.1∶1,respectively. Under the optimized conditions, after 25 min of adsorption, the CV current linearly responded. to the logarithm of CoQ10 concentration within the range of 0.025-25.0 μmol/L, with a regression equation of ,I, = 2.554 + 1.479lg,C,(,r,2, = 0.997), and a detection limit (LOD)of 19.0 nmol/L. The detection of CoQ10 by using adsorptive CV on GCE could achieve its accurate and high response quantitative analysis.
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