CHEN Ya,ZHANG Jiang-qun,CHANG Zi-han,WANG Tao,HU Meng,ZHANG Yan,LONG Xing-yu.Rapid Detection of Phosphate in Different Water Samples by Digital Colourimetric Method with Smartphone[J].Journal of Instrumental Analysis,2025,44(07):1-7.
CHEN Ya,ZHANG Jiang-qun,CHANG Zi-han,WANG Tao,HU Meng,ZHANG Yan,LONG Xing-yu.Rapid Detection of Phosphate in Different Water Samples by Digital Colourimetric Method with Smartphone[J].Journal of Instrumental Analysis,2025,44(07):1-7. DOI: 10.12452/j.fxcsxb.241101504.
Rapid Detection of Phosphate in Different Water Samples by Digital Colourimetric Method with Smartphone
A reduction in the level of phosphate in water bodies will result in the eutrophication of these ecosystems,which represents a significant threat to the ecological environment. Moreover,the existing phosphate detection technology is costly,time-consuming,and unable to meet the requirements for rapid detection and other pertinent criteria. A smartphone digital colorimetry (SDC) method based on phosphomolybdenum blue colorimetry was developed for the rapid determination of phosphate content in water bodies. The impact of diverse smartphone models,RGB values,shooting magnification,light source type and light intensity on the outcomes of SDC was examined in the experiments. The findings illustrated a favourable linear correlation between the red value(
R
-value) of phosphate and the HUAWEI P50e mobile phone within the concentration range of 0-800 μg·L
-1
,with the
R
2
value of 0.9976. The variables included the red-channel value as the assay value,3× filming magnification,a white light source,and a high light intensity condition. The limit of detection of the method was 1.63 μg·L
-1
and the limit of determination was 4.89 μg·L
-1
. Under the optimized conditions,two different concentrations of phosphate solutions,with the 100 μg·L
-1
and 400 μg·L
-1
,were measured 10 times in parallel and the relative standard deviations(RSDs) were calculated to be 0.35% and 0.31%,respectively. Morever,the 400 μg·L
-1
phosphate were further examined with intraday/interday RSDs(
n
=5) of 0.38% and 0.42%,respectively. The SDC method was employed for the determination of phosphate in four distinct real water bodies(tap water,rainwater,Siya River water and Baiyun Lake water),with spiked recoveries ranging from 96.1% to 101%. This indicates that the method is both feasible and accurate,providing a novel approach for th
e rapid determination of phosphate in aquatic environments in the field.
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references
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