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1.南阳市产品质量检验检测中心,河南 南阳 473003
2.南阳医学高等专科学校,河南 南阳 473061
3.河南福森药业有限公司,河南 南阳 474450
Received:28 August 2024,
Revised:23 September 2024,
Accepted:2024-11-06,
Published:15 April 2025
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陈林,黄涵,赵培敬,李中娥,宋颖,方朝清.基于UHPLC-Q-Orbitrap-HRMS技术比较金银花与忍冬枝条的化学成分[J].分析测试学报,2025,44(04):645-651.
CHEN Lin,HUANG Han,ZHAO Pei-jing,LI Zhong-e,SONG Ying,FANG Chao-qing.Comparison of Chemical Components of Lonicerae Japonicae Flos and Lonicerae Japonicae Branches by UHPLC-Q-Orbitrap-HRMS[J].Journal of Instrumental Analysis,2025,44(04):645-651.
陈林,黄涵,赵培敬,李中娥,宋颖,方朝清.基于UHPLC-Q-Orbitrap-HRMS技术比较金银花与忍冬枝条的化学成分[J].分析测试学报,2025,44(04):645-651. DOI: 10.12452/j.fxcsxb.240828351.
CHEN Lin,HUANG Han,ZHAO Pei-jing,LI Zhong-e,SONG Ying,FANG Chao-qing.Comparison of Chemical Components of Lonicerae Japonicae Flos and Lonicerae Japonicae Branches by UHPLC-Q-Orbitrap-HRMS[J].Journal of Instrumental Analysis,2025,44(04):645-651. DOI: 10.12452/j.fxcsxb.240828351.
该文基于超高效液相色谱
-
四极杆/静电场轨道阱高分辨质谱(UHPLC
-
Q
-
Orbitrap
-
HRMS)技术比较金银花与忍冬枝条的化学成分。首先通过高效液相色谱(HPLC)技术结合指纹图谱分析不同采收期忍冬枝条与金银花的化学成分相似度,结果表明10月底采收的忍冬枝条与金银花的相似度最高,达到85%。然后在电喷雾正、负电离模式下,采用UHPLC-Q-Orbitrap-HRMS技术对10月底采收的忍冬枝条与金银花提取物进行全扫描及二级质谱扫描,通过与对照品比较、在线数据库匹配及文献检索,共鉴定出38种化学成分,其中35种为共有成分。将35种共有成分的质谱数据导入MetaboAnalyst网站,基于偏最小二乘分析算法计算变量投影重要性(VIP)分析值,结果有8个化合物的VIP
>
1,表明10月底采收的忍冬枝条与金银花的化学成分相似度较高,差异性主要体现在部分成分的含量上。该研究可为忍冬枝条资源的合理开发利用提供有效的数据
支撑。
The chemical component of
Lonicerae japonicae
Flos and
Lonicerae japonicae
branches was compared based on the utilizing ultra-high-performance quadrupole-orbitrap high-resolution mass spectrometry(UHPLC-Q-Orbitrap-HRMS) technique. Firstly,the similarity of chemical components between
Lonicerae japonicae
branches and
Lonicerae japonicae
flos at different harvesting periods was analysed by high-performance liquid chromatography(HPLC) technique combined with fingerprinting,and the results showed that
Lonicerae japonicae
branches harvested at the end of October had the highest similarity with honeysuckle,reaching 85%. Then UHPLC-Q-Orbitrap-HRMS technology was used to conduct full scanning and secondary mass spectrometry scanning on the extracts of
Lonicerae japonicae
flos and
Lonicerae japonicae
branches at the end of October in positive and negative ionisation modes,and 38 chemical components were identified by comparison with reference substance,online database matching and literature search,among which 35 were common components. The mass spectrometry data of 35 common components were imported into the MetaboAnalyst website,and the projected importance value(VIP) of variables was calculated based on the partial least squares analysis algorithm. As a result,there were eight compounds with VIP
>
1,indicating that the chemical composition of
Lonicerae japonicae
branches harvested at the end of October had a high degree of similarity with that of
Lonicerae japonicae
flos,and the differences were mainly reflected in the contents of some components. The study can provide effective data support for the rational development and utilization of
Lonicerae japonicae
branches resources.
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