Determination of 23 Laxative Ingredients Including Oxyphenisatine in Food and Health-care Food by UPLC-MS/MS

ZHANG Qiu-yan; LUO Hui-tai; LIAO Jun-tao; HUANG Fang; LIANG Wei-wei; WU Hui-qin

doi:10.12452/j.fxcsxb.240705194

您当前的位置：

首页 >

文章列表页 >

Determination of 23 Laxative Ingredients Including Oxyphenisatine in Food and Health-care Food by UPLC-MS/MS

Analysis and Testing Summit Forum Call for Papers Column | 更新时间：2025-02-12

- Determination of 23 Laxative Ingredients Including Oxyphenisatine in Food and Health-care Food by UPLC-MS/MS
- Journal of Instrumental Analysis Vol. 44, Issue 2, Pages: 211-220(2025)
- 作者机构：
  
  广东省科学院测试分析研究所（中国广州分析测试中心），广东省化学测量与应急检测技术重点实验室，广东省中药质量安全工程技术研究中心，广东广州 510070
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.240705194
  CLC： O657.7;TS207.3
- Published：15 February 2025，
  
  Received：05 July 2024，
  
  Revised：14 August 2024，
  
  Accepted：2024-09-29
- 稿件说明：
移动端阅览
张秋炎,罗辉泰,廖均涛,黄芳,梁维维,吴惠勤.UPLC-MS/MS测定食品及保健食品中酚汀等23种致泻性成分[J].分析测试学报,2025,44(02):211-220.

ZHANG Qiu-yan,LUO Hui-tai,LIAO Jun-tao,HUANG Fang,LIANG Wei-wei,WU Hui-qin.Determination of 23 Laxative Ingredients Including Oxyphenisatine in Food and Health-care Food by UPLC-MS/MS[J].Journal of Instrumental Analysis,2025,44(02):211-220.
张秋炎,罗辉泰,廖均涛,黄芳,梁维维,吴惠勤.UPLC-MS/MS测定食品及保健食品中酚汀等23种致泻性成分[J].分析测试学报,2025,44(02):211-220. DOI： 10.12452/j.fxcsxb.240705194.

ZHANG Qiu-yan,LUO Hui-tai,LIAO Jun-tao,HUANG Fang,LIANG Wei-wei,WU Hui-qin.Determination of 23 Laxative Ingredients Including Oxyphenisatine in Food and Health-care Food by UPLC-MS/MS[J].Journal of Instrumental Analysis,2025,44(02):211-220. DOI： 10.12452/j.fxcsxb.240705194.

摘要

建立了同时测定食品及保健食品中非法添加酚汀等23种致泻性成分的超高效液相色谱-串联质谱法（UPLC-MS/MS）。样品经80%甲醇（含0.1%甲酸）提取后，提取液在0.2%甲酸溶液-乙腈的流动相体系下经Agilent Poroshell 120 PFP（2.1 mm×100 mm，2.7 μm）色谱柱梯度洗脱分离。采用电喷雾正离子模式下多反应监测（MRM）方式对酚汀等23种致泻性成分进行质谱分析，基质匹配外标法定量。实验表明，酚汀等23种致泻性成分在不同基质（饮料、压片糖果、硬胶囊内容物、果蔬粉、代用茶、酵素梅和果冻）中线性关系良好，相关系数（

）为0.997 1~0.999 9，检出限为0.001~0.5 mg/kg，定量下限为0.002~1.0 mg/kg。饮料、压片糖果、硬胶囊内容物、果蔬粉、代用茶、酵素梅和果冻样品在3个不同加标水平下的平均回收率为77.5%~114%，相对标准偏差为0.81%~12%（

=6）。该法前处理易操作且高效，分析速度快，灵敏度高，定量准确，适用于食品及保健食品中酚汀等23种致泻性成分的同时快速分析。

Abstract

A novel method for the simultaneous and rapid determination of 23 laxative ingredients including oxyphenisatine in various food and health-care food matrices using ultra high performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） has been developed. The different matrix samples were extracted with 80% methanol（containing 0.1%

formic acid），and the filtrate was analyzed using an Agilent Poroshell 120 PFP chromatographic column（2.1 mm×100 mm，2.7 μm）. The mobile phases were 0.2% formic acid aqueous solution and acetonitrile under gradient elution. The mass spectrometry conditions employed an electrospray ionization source and positive ion scanning with multiple reaction monitoring mode（MRM） for 23 laxative ingredients including oxyphenisatine. Representative matrices such as drink，tablet candy，hard capsule contents，fruit and vegetable powder，substitutional tea，enzymatic plum and jelly samples ware selected. The correlation coefficients（

） for linear calibration curves of 23 laxative ingredients ranged from 0.997 1 to 0.999 9 in their respective concentration range. The limits of detection（LODs） of 23 laxative ingredients were in the range of 0.001-0.5 mg/kg，and the limits of quantitation（LOQs） were in the range of 0.002-1.0 mg/kg. The recoveries at low，medium and high concentration levels ranged from 77.5% to 114%，with relative standard deviations（

=6） ranged from 0.81% to 12%. The established method is simple，effective，rapid，high sensitive and accurate，making it suitable for the determination of 23 laxative ingredients in food and health-care food.

关键词

超高效液相色谱-串联质谱法食品保健食品酚汀酚酞致泻性成分

Keywords

UPLC-MS/MSfoodhealth-care foodoxyphenisatinephenolphthaleinlaxative ingredients

references

Ma J R，Wang Z Y，Sun M C，Liu S Y，Xu R J，Sun X M，Peng W，Wang Y F. J. Prevent. Med.（马靖茹，王智奕，孙美晨，刘时雨，许睿洁，孙晓敏，彭雯，王友发. 中国预防医学杂志），2024，25（4）：406-412.

He Y，Zhao X L，Zeng Q. Prart. Prev. Med.（贺媛，赵小兰，曾强. 实用预防医学），2015，22（4）：390-394.

Liu Q，Du Y，Yang L，Wu G Q. Sci. Technol. Food Ind.（刘齐，杜勇，杨玲，邬国庆. 食品工业科技），2019，40（1）：209-213.

Wang A Z，Ni Y X. J. Liaoning Police Coll.（王爱芝，倪玉霞. 辽宁警察学院学报），2023，25（3）：69-75.

Ji W X，Pan H B，Liu D. J. Shanxi Polit. Law Inst. Adm.（纪文旭，潘虹伯，刘丹. 山西省政法管理干部学院学报），2023，36（4）：76-80.

Zhang L，Li K Q，Zhu H，Zhao M J，Guan X，Liu H Z，Wu Q. J. Food Saf. Food Qual.（张璐，李可强，朱辉，赵美瑾，关昕，刘洪喆，吴琼. 食品安全质量检测学报），2021，12（3）：904-913.

Ma C Y，Duan Q，Gao J M，Feng S H. J. Food Saf. Food Qual.（马春艳，段琼，高晋梅，封淑华. 食品安全质量检测学报），2021，12（12）：4998-5006.

BJS 201701. National Medical Products Administration. Determination of Sibutramine and Other Compounds in Food （国家食品药品监督管理总局. 食品中西布曲明等化合物的测定）.

BJS 202209. National Medical Products Administration. Determination of Oxyphenisatine Di（acetate） and Other 18 Compounds in Food （国家食品药品监督管理总局. 食品中双醋酚丁等19种化合物的测定）.

Liu S L. China Food Drug Adm. Mag. （刘素丽. 中国食品药品监管），2022，（8）：90-95.

State Administration for Market Regulation. Document No.〔2023〕94 of the General Office of the State Administration for Market Regulation （国家市场监督管理总局. 国家市场监督管理总局办公厅文件市监稽发〔2023〕94号）. https：//www.samr.gov.cn/zw/zfxxgk/fdzdgknr/zfjcs/art/2023/art_5b69a28fde634bfab1dc178a63fce009.htmlhttps://www.samr.gov.cn/zw/zfxxgk/fdzdgknr/zfjcs/art/2023/art_5b69a28fde634bfab1dc178a63fce009.html.

State Administration for Market Regulation. Document No.〔2023〕95 of the General Office of the State Administration for Market Regulation. Determination of Oxyphenisatin Dipropionate in Food

国家市场监督管理总局. 国家市场监督管理总局办公厅文件市监稽发〔2023〕95号. 食品中双丙酚汀的测定方法.

Guangdong Administration for Market Regulation. Document No. 〔2023〕 456 of Guangdong Administration for Market Regulation. Determination of Oxyphenisatine Compounds in Food （广东省市场监督管理局. 粤市监协调〔2023〕456号. 食品中酚汀类物质的测定）.

Li J，Li Y Y，Wen L Y. Tianjin Pharm.（李静，李倚云，闻琍毓. 天津药学），2015，27（2）：17-18.

Wang H，Yang Q，Wan Y，Zhao X Y，Shang Y Z，Wang H，Wang P. Port Sci. Technol.（王晗，杨青，万莹，赵晓亚，尚吟竹，王惠，王鹏. 中国口岸科学技术），2023，5（7）：47-55.

Xun Z Q，He S，Wang C X，Wu C S，Huang J F，Guo X D. Guangdong Chem. Ind.（寻知庆，何霜，汪晨霞，吴楚森，黄金凤，郭新东. 广东化工），2021，48（24）：153-155.

Mao M，Yang Z J. Fujian Anal. Test.（毛敏，杨志金. 福建分析测试），2023，32（6）：29-32.

Yuan Y L，Yuan L J，Guo L J，Shi L. Mod. Food Sci. Technol.（袁阳蕾，袁利杰，郭立净，石璐. 现代食品科技），2023，39（1）：311-319.

Qiu Y J，Jia Y B，Jiang H，Sun L，Chen M C，Chen L F，Yu J，Lin S Y. Bull. Ferment. Sci. Technol.（裘一婧，贾彦博，江海，孙岚，陈美春，陈丽芳，余菁，林舒忆. 发酵科技通讯），2024，53（1）：1-7.

Huang K H，Luo X，Zhou X，Wang W F，Yang F X，Zhu G F，Sun Y. Food Sci. Technol.（黄康惠，卢鑫，周璇，王文富，杨福兴，朱桂芳，孙宇. 食品科技），2023，48（6）：301-307.

Xia J T，Wu W Q，Zhu S S，Jiang F. Food Mach.（夏金涛，吴婉琴，朱松松，江丰. 食品与机械），2024，40（2）：21-27.

EC. Commission Decision EC/657/2002 of 12 August 2002 Implementing Council Directive 96/23/EC Concerning the Performance of Analytical Methods and the Interpretation of Results. Offic. J. Eur. Comm.，2002.［2002-08-17］. http：//eur-lex.europa.eu/legal-content/EN/ALL/？uri=CELEX：32002D0657http://eur-lex.europa.eu/legal-content/EN/ALL/?uri=CELEX:32002D0657.

Luo H T，Huang X L，Wu H Q，Zhang Q Y，Zhu Z X，Huang F，Lin X S，Ma Y F，Deng X. Chin. J. Chromatogr.（罗辉泰，黄晓兰，吴惠勤，张秋炎，朱志鑫，黄芳，林晓珊，马叶芬，邓欣. 色谱），2017，35（8）：816-825.

Zhang Q Y，Liang W W，Luo H T，Liao J T，Huang F，Wu H Q. J. Instrum. Anal. （张秋炎，梁维维，罗辉泰，廖均涛，黄芳，吴惠勤. 分析测试学报），2024，43（2）：285-291.

Zhang Q Y，Huang F，Liang W W，Liao J T，Wu H Q，Luo H T. J. Instrum. Anal. （张秋炎，黄芳，梁维维，廖均涛，吴惠勤，罗辉泰. 分析测试学报），2024，43（5）：663-673.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Advance in Chromatographic Techniques for Polycyclic Aromatic Hydrocarbons Detection in Food

Research on the Determination Method of Ethephon Residue in Traditional Chinese Medicine

Related Author

FENG Gui-rong

LI Hong

HUANG Xian-liang

HUANG Dan-dan

CHEN Li-wei

CAI Xiao-qin

HUANG Si-yu

HOU Mei-ling

Related Institution

Chongqing Institute for Food and Drug

Control Key Laboratory of State Market Regulation（Condiment Supervision Technology）

School of Pharmacy，Shanghai University of Traditional Chinese Medicine

National Medical Products Administration Key Laboratory for Quality Control of Traditional Chinese Medicine，Shanghai Institute for Food and Drug Control

Nanchang Hongdu Hospital of Traditional Chinese Medicine

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
Total Visits：380137 Visits Today：615

⁰