Investigation on the Bioaccumulation and Tissue Distribution of Per- and Polyfluoroalkyl Substances in Zebrafish by UPLC-MS/MS

ZENG Hai-shen; HE Cai; YANG Yun-yun; ZENG Xi-liu; XIE Jia-liang; LIU Ning; DENG Jie-wei; LUAN Tian-gang

doi:10.12452/j.fxcsxb.24052669

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Investigation on the Bioaccumulation and Tissue Distribution of Per- and Polyfluoroalkyl Substances in Zebrafish by UPLC-MS/MS

Scientific Papers | 更新时间：2024-08-08

- Investigation on the Bioaccumulation and Tissue Distribution of Per- and Polyfluoroalkyl Substances in Zebrafish by UPLC-MS/MS
- Journal of Instrumental Analysis Vol. 43, Issue 8, Pages: 1189-1196(2024)
- 作者机构：
  
  1.化学与精细化工广东省实验室揭阳分中心（广东省榕江实验室），广东揭阳 515200
  2.广东工业大学生物医药学院，广东广州 510006
  3.广东省科学院测试分析研究所（中国广州分析测试中心），广东广州 510070
  4.五邑大学环境与化学工程学院，广东江门 529020
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.24052669
  CLC： O657.7;X132
- Published：15 August 2024，
  
  Received：26 May 2024，
  
  Revised：27 June 2024，
- 稿件说明：
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曾海深,何财,杨运云,曾细柳,谢嘉良,刘宁,邓洁薇,栾天罡.基于UPLC-MS/MS的全氟及多氟烷基化合物在斑马鱼体内的生物富集与组织分布研究[J].分析测试学报,2024,43(08):1189-1196.

ZENG Hai-shen,HE Cai,YANG Yun-yun,ZENG Xi-liu,XIE Jia-liang,LIU Ning,DENG Jie-wei,LUAN Tian-gang.Investigation on the Bioaccumulation and Tissue Distribution of Per- and Polyfluoroalkyl Substances in Zebrafish by UPLC-MS/MS[J].Journal of Instrumental Analysis,2024,43(08):1189-1196.
曾海深,何财,杨运云,曾细柳,谢嘉良,刘宁,邓洁薇,栾天罡.基于UPLC-MS/MS的全氟及多氟烷基化合物在斑马鱼体内的生物富集与组织分布研究[J].分析测试学报,2024,43(08):1189-1196. DOI： 10.12452/j.fxcsxb.24052669.

ZENG Hai-shen,HE Cai,YANG Yun-yun,ZENG Xi-liu,XIE Jia-liang,LIU Ning,DENG Jie-wei,LUAN Tian-gang.Investigation on the Bioaccumulation and Tissue Distribution of Per- and Polyfluoroalkyl Substances in Zebrafish by UPLC-MS/MS[J].Journal of Instrumental Analysis,2024,43(08):1189-1196. DOI： 10.12452/j.fxcsxb.24052669.

摘要

采用超高效液相色谱-串联质谱（UPLC-MS/MS）研究了全氟及多氟烷基化合物（PFASs）在斑马鱼体内的生物富集与组织分布。以全氟辛烷磺酸（PFOS）、全氟辛酸（PFOA）、全氟己烷磺酸（PFHxS）和全氟癸酸（PFDA）对斑马鱼联合暴露7、21、28 d，暴露浓度为100 μg/L。斑马鱼不同组织器官分离后，用甲醇进行提取，以UPLC-MS/MS进行检测，负离子多反应监测（MRM）模式进行定量测定。结果表明，4种PFASs在1~200 μg/L范围内线性关系良好，相关系数（

）不小于0.997 4，检出限（LOD）和定量下限（LOQ）分别为0.072~0.152 ng/g和0.287~0.609 ng/g。斑马鱼暴露于4种PFASs后，其体内各器官均分布有上述4种污染物。其中PFDA和PFOS在暴露7、21、28 d的斑马鱼体内器官的积累程度相对较高；PFOA主要在肠道和肝脏中的积累量较高；PFHxS在斑马鱼体内的积累量最低，在各器官中的含量对4种污染物含量总和的相对贡献均低于13%。该研究为探索PFASs在水生生物体内的环境行为和生态风险提供了科学依据。

Abstract

A method for analysis of the bioaccumulation and tissue distribution of per- and polyfluoroalkyl substances（PFASs） in zebrafish using ultra high performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） was established. Zebrafishes were exposed to perfluorooctane sulfonate（PFOS），perfluorooctanoic acid（PFOA），perfluorohexanesulfonic acid（PFHxS），and perfluorohexanesulfonic acid（PFDA） for durations of 7，21，and 28 days at a concentration of 100 μg/L for each analyte. After exposure，various tissues and organs from the zebrafish were isolated，

extracted with methanol，and subsequently analyzed using UPLC-MS/MS under negative ion multiple reaction monitoring（MRM） mode. The results showed that there were good linear relationships in the range of 1-200 μg/L for four PFASs，with a correlation coefficient（

） not less than 0.997 4. The limits of detection（LODs） and limits of quantitation（LOQs） of the method were 0.072-0.152 ng/g and 0.287-0.609 ng/g，respectively. After being exposed to four kinds of PFASs，the zebrafish's organs will all be polluted by these four pollutants. Among the four PFASs，both PFDA and PFOS showed relatively higher accumulation levels in the organs of zebrafish. PFOA accumulates mainly in high amounts in the intestine and liver. PFHxS shows the lowest accumulation in zebrafish，with its relative contribution to the total content of the four pollutants in various organs being less than 13%. This study provides a scientific basis for elucidating the environmental behavior and ecological risk of PFASs within aquatic organisms.

关键词

超高效液相色谱-串联质谱斑马鱼全氟及多氟烷基化合物生物富集组织分布

Keywords

UPLC-MS/MSzebrafishPFASsbioaccumulationtissue distribution

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