Study on the Effects of the Ambient Fine Particulate Matter （PM2.5） Exposure on Urinary Metabolic Profiles in Rats Using UPLC-Q-TOF MS

LU Ting-ting; QIN Peng; XUE Hui-ming; FENG Zhen; LUO Xu-ping; ZHENG Mei-zhu

doi:10.12452/j.fxcsxb.24042804

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Study on the Effects of the Ambient Fine Particulate Matter （PM2.5） Exposure on Urinary Metabolic Profiles in Rats Using UPLC-Q-TOF MS

Scientific Papers | 更新时间：2024-08-08

- Study on the Effects of the Ambient Fine Particulate Matter （PM2.5） Exposure on Urinary Metabolic Profiles in Rats Using UPLC-Q-TOF MS
- Journal of Instrumental Analysis Vol. 43, Issue 8, Pages: 1294-1300(2024)
- 作者机构：
  
  1.长春师范大学生命科学学院，吉林长春 130032
  2.长春师范大学中心实验室，吉林长春 130032
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.24042804
  CLC： O657.63;X132
- Published：15 August 2024，
  
  Received：28 April 2024，
  
  Revised：14 June 2024，
- 稿件说明：
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鲁婷婷,秦鹏,薛慧铭,冯真,罗旭萍,郑梅竹.基于UPLC-Q-TOF MS的染毒（PM2.5）大鼠尿液代谢组学研究[J].分析测试学报,2024,43(08):1294-1300.

LU Ting-ting,QIN Peng,XUE Hui-ming,FENG Zhen,LUO Xu-ping,ZHENG Mei-zhu.Study on the Effects of the Ambient Fine Particulate Matter （PM2.5） Exposure on Urinary Metabolic Profiles in Rats Using UPLC-Q-TOF MS[J].Journal of Instrumental Analysis,2024,43(08):1294-1300.
鲁婷婷,秦鹏,薛慧铭,冯真,罗旭萍,郑梅竹.基于UPLC-Q-TOF MS的染毒（PM2.5）大鼠尿液代谢组学研究[J].分析测试学报,2024,43(08):1294-1300. DOI： 10.12452/j.fxcsxb.24042804.

LU Ting-ting,QIN Peng,XUE Hui-ming,FENG Zhen,LUO Xu-ping,ZHENG Mei-zhu.Study on the Effects of the Ambient Fine Particulate Matter （PM2.5） Exposure on Urinary Metabolic Profiles in Rats Using UPLC-Q-TOF MS[J].Journal of Instrumental Analysis,2024,43(08):1294-1300. DOI： 10.12452/j.fxcsxb.24042804.

摘要

为探明PM2.5对大鼠代谢组学的影响，将SD大鼠随机分为对照组（SC）和PM2.5模型组（MG），每组15只。将生理盐水和PM2.5混悬液分别注入各组大鼠气管，每周2次，持续4周。通过超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF MS）对各组大鼠尿液的代谢组学变化进行检测，采用多元统计分析探究整体代谢组学的变化。通过数据分析和数据库检索，从大鼠尿样中鉴定出17种潜在生物标记物，主要代谢途径涉及戊糖和葡萄糖醛酸相互转化、色氨酸代谢、酪氨酸代谢、苯丙氨酸代谢、嘌呤代谢、对乙酰氨基酚代谢、视黄醇代谢和丙戊酸代谢途径，PM2.5对大鼠诱导损伤作用，可能与其扰乱大鼠体内正常代谢活动有关。研究结果有助于了解PM2.5的毒理学机制，筛选PM2.5暴露大鼠的潜在生物标志物，为进一步探索PM2.5毒性作用及致病机制提供了理论依据。

Abstract

To investigate the effects of PM2.5 on metabolomics in rats，SD rats were randomly divided into a control group（SC） and a PM2.5 model group（MG），with 15 rats in each group. Saline and PM2.5 suspension were injected into the trachea of rats in each group twice a week for 4 weeks，respectively. The metabolomic changes in the urine of rats in each group were examined by ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry（UPLC-Q-TOF MS），and multivariate statistical analyses were performed to explore the overall metabolomic changes. Through data analysis and database search，17 potential biomarkers were identified from rat urine samples，and the main metabolic pathways involved pentose and glucuronide interconversion，tryptophan metabolism，tyrosine metabolism，phenylalanine metabolism，purine metabolism，acetaminophen metabolism pathway，retinol metabolism and valproate metabolism pathway. PM2.5 induced damaging effects in the rats，which may be related to its disruption of normal metabolic activities in rats. The results of the present study can help to understand the toxicological mechanism of PM2.5，screen the potential biomarkers of PM2.5-exposed rats，and provide theoretical bases for further exploration of the toxic effects and pathogenic mechanisms of PM2.5.

关键词

超高效液相色谱-四极杆-飞行时间质谱PM2.5代谢组学尿液

Keywords

UPLC-Q-TOF MSambient fine particulate mattermetabolomicsurine

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