Sensitive Detection of Telomerase Activity Based on a G-quadruplex Electrochemiluminescent Biosensor

HE Zhang-jin; DU Yu-ying; LU Li-ping

doi:10.12452/j.fxcsxb.24041803

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Sensitive Detection of Telomerase Activity Based on a G-quadruplex Electrochemiluminescent Biosensor

Scientific Papers | 更新时间：2024-07-08

- Sensitive Detection of Telomerase Activity Based on a G-quadruplex Electrochemiluminescent Biosensor
- Journal of Instrumental Analysis Vol. 43, Issue 7, Pages: 963-970(2024)
- 作者机构：
  
  北京工业大学环境科学系，北京 100124
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.24041803
  CLC： O646;TP212.3
- Published：15 July 2024，
  
  Received：18 April 2024，
  
  Revised：06 May 2024，
- 稿件说明：
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贺彰瑾,杜雨莹,鲁理平.基于G-四链体电化学发光生物传感器灵敏检测端粒酶活性[J].分析测试学报,2024,43(07):963-970.

HE Zhang-jin,DU Yu-ying,LU Li-ping.Sensitive Detection of Telomerase Activity Based on a G-quadruplex Electrochemiluminescent Biosensor[J].Journal of Instrumental Analysis,2024,43(07):963-970.
贺彰瑾,杜雨莹,鲁理平.基于G-四链体电化学发光生物传感器灵敏检测端粒酶活性[J].分析测试学报,2024,43(07):963-970. DOI： 10.12452/j.fxcsxb.24041803.

HE Zhang-jin,DU Yu-ying,LU Li-ping.Sensitive Detection of Telomerase Activity Based on a G-quadruplex Electrochemiluminescent Biosensor[J].Journal of Instrumental Analysis,2024,43(07):963-970. DOI： 10.12452/j.fxcsxb.24041803.

摘要

端粒酶活性的分析检测对于癌症诊断、预后治疗等具有重要意义。该研究利用G-四链体（G4） DNA的金属配合物靶向发光探针［Ir（ppy）

（pip）］PF

，构建了灵敏检测癌细胞端粒酶活性的电化学发光（ECL）生物传感器。首先将引物DNA组装在电极表面，然后

与癌细胞提取的端粒酶共同孵育，具有活性的端粒酶在引物末端生成端粒重复序列从而形成G4结构，随后［Ir（ppy）

（pip）］PF

通过与G4靶向作用结合到延长的序列中。结果表明［Ir（ppy）

（pip）］PF

的ECL信号与端粒酶活性呈现正相关，传感器的电化学发光强度与癌细胞浓度在10 ~ 5×10

cell/mL呈现良好的线性关系，检出限（LOD）为3 cell/mL。实验还表明了传感器具有良好的稳定性、适用性、抗干扰性和重现性，最后将该传感器用于测定真实人类血清和尿液样本中的端粒酶活性，得到了满意的结果。

Abstract

The analysis and detection of telomerase activity is important for cancer diagnosis，prognosis，and treatment. In this study，an electrochemiluminescence（ECL） biosensor was constructed for sensitive detection of telomerase activity in cancer cells using a metal complex-targeted luminescent probe［Ir（ppy）

（pip）］PF

for G-quadruplex（G4） DNA. Primer DNA was first assembled on the electrode surface and then co-incubated with telomerase extracted from cancer cells. The active telomerase generated telomeric repeats at the end of the primer to form the G4 structure，and then［Ir（ppy）

（pip）］PF

bound to the extended sequence by targeting with G4. The results showed that the ECL signal of［Ir（ppy）

（pip）］PF

showed a positive correlation with telomerase activity，and the ECL intensity of the sensor showed a good linear correlation with the concentration of cancer cells in the range of 10-5×10

cell/mL，with the limit of detection（LOD） of 3 cell/mL. The experiments also demonstrated that the sensor had good stability，applicability，anti-interference ablity，and reproducibility. Finally，the sensor was used for the determination of telomerase activity in real human serum and urine samples，and satisfactory results were also obtained.

关键词

端粒酶活性G-四链体金属配合物电化学发光生物传感器

Keywords

telomerase activityG-quadruplexmetal complexelectrochemiluminescencebiosensor

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