CHEN Yu-xin,HU Yue-fang.Preparation and Application of Nitrogen-doped Carbon Quantum Dots in Cell Temperature Sensing[J].Journal of Instrumental Analysis,2024,43(04):566-573.
CHEN Yu-xin,HU Yue-fang.Preparation and Application of Nitrogen-doped Carbon Quantum Dots in Cell Temperature Sensing[J].Journal of Instrumental Analysis,2024,43(04):566-573. DOI: 10.12452/j.fxcsxb.23111301.
Preparation and Application of Nitrogen-doped Carbon Quantum Dots in Cell Temperature Sensing
In this study,nitrogen self-doped carbon quantum dots(N-CQDs) were prepared by microwave method using biomass tomato as carbon and nitrogen sources. The morphology,structure,stability,photoelectric properties,temperature sensitivity and cytotoxicity were studied by field emission transmission electron microscope(TEM),X-ray photoelectron spectrometer(XPS) and fluorescence luminescence photometer,and were applied to temperature sensing in living cells. The experimental results show that the prepared N-CQDs has strong stability,excellent photoelectric performance and strong sensitivity to temperature. In the temperature range of 25-65 ℃,the fluorescence intensity of N-CQDs decreases with the increase of temperature,and shows a good linear relationship. The N-CQDs can penetrate the cell membrane of 4T1 cells,showing low toxicity and biocompatibility,and good temperature sensing fluorescence properties in 4T1 cells. It is indicated that the N-CQDs has great potential and value in the field of fluorescence temperature sensing in living cells,and is of great significance in the development of non-destructive cell measurement of nanoferters. It is expected to become a temperature-sensitive fluorescence nanoprobe and be widely used in biomedical fields such as cell research.
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