Synthesis and Characterization of a Long-term Stable Lead-dependent DNAzyme

CHEN Ci; KUAI Hai-lan; LI Lin-ling; HU Xia; YANG Ji-feng; ZHANG Song-bai

doi:10.12452/j.fxcsxb.23083104

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Synthesis and Characterization of a Long-term Stable Lead-dependent DNAzyme

Scientific Papers | 更新时间：2024-01-17

- Synthesis and Characterization of a Long-term Stable Lead-dependent DNAzyme
- Journal of Instrumental Analysis Vol. 43, Issue 1, Pages: 191-199(2024)
- 作者机构：
  
  湖南文理学院，化学与材料工程学院，水处理功能材料湖南省重点实验室，电镀废水回用技术湖南省工程研究中心湖南常德 415000
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.23083104
  CLC： O657.3;O614.433
- Published：15 January 2024，
  
  Received：31 August 2023，
  
  Revised：20 November 2023，
- 稿件说明：
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陈词,蒯海岚,李林羚等.一种长时稳定的铅离子响应型脱氧核酶的合成与表征[J].分析测试学报,2024,43(01):191-199.

CHEN Ci,KUAI Hai-lan,LI Lin-ling,et al.Synthesis and Characterization of a Long-term Stable Lead-dependent DNAzyme[J].Journal of Instrumental Analysis,2024,43(01):191-199.
陈词,蒯海岚,李林羚等.一种长时稳定的铅离子响应型脱氧核酶的合成与表征[J].分析测试学报,2024,43(01):191-199. DOI： 10.12452/j.fxcsxb.23083104.

CHEN Ci,KUAI Hai-lan,LI Lin-ling,et al.Synthesis and Characterization of a Long-term Stable Lead-dependent DNAzyme[J].Journal of Instrumental Analysis,2024,43(01):191-199. DOI： 10.12452/j.fxcsxb.23083104.

摘要

脱氧核酶作为一类具有酶活性的功能核酸分子，以其不同于蛋白酶和核酶的结构优势而被广泛应用于生物传感等分析检测领域。但核酸结构易受生物酶降解，故而限制了脱氧核酶在该领域应用研究中的推进。化学修饰的方法可改善该问题，但仍存在修饰繁琐且价格昂贵等弊端。该文基于核酸结构改造，利用环形核酸结构的耐酶切能力，设计合成了一种环状结构的铅离子响应型脱氧核酶，并对其性能进行了表征。研究结果表明该环形脱氧核酶在外切酶样、自来水样以及75%胎牛血清（FBS）中具有比传统单链脱氧核酶更好的结构稳定性，其中在75%胎牛血清中稳定存在的时长达6 h。此外，环形结构的引入也增强了脱氧核酶与底物序列的相互作用，进而影响基于环形脱氧核酶的生物传感器的信号表达，这一特性有望为脱氧核酶生物传感器的构建提供新思路。

Abstract

As a type of functional nucleic acid with enzymatic activity，DNAzymes are widely used in analytical fields such as biosensing due to their structural advantages that are different from proteases and ribozymes. However，the structure of DNAzyme is susceptible to degradation by biological enzymes，which slows down the speed of such biosensor development. Although chemical modification can be used to resolve the problem，this strategy has disadvantages，such as being costly and hard to synthesize. Considering this，we choose a new strategy from the sights of structure transformation to resolve this problem. Then，we design and synthesize a kind of circular DNAzyme by utilizing the enzyme-resistance properties of circular nucleic acid structure. The research results show that the circular DNAzyme has better structural stability than traditional single-stranded DNAzymes in exonuclease，tap water，and 75% fetal bovine serum（FBS），and the duration is up to 6 h in 75% FBS. In addition，introducing a circular structure will also enhance the interaction between DNAzymes and substrate sequences，thereby affecting the signal expression of biosensors based on circular DNAzymes. This feature will provide new ideas for constructing DNAzyme-based biosensors.

关键词

环形脱氧核酶环形核酸脱氧核酶铅离子检测生物传感器

Keywords

cicular DNAzymecicular nucleic acidDNAzymePb2+ detectionbiosensor

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Related Institution

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