In Situ Synthesis of Au/Foam Nickel Magnetic Substrate for Rapid Detection of Immunosuppressive Drug Metabolites in Serum by Using Surface Enhanced Raman Scattering Spectroscopy

LIU Yao-yao; LI Duo-duo; GUO Xiao-yu; WEN Ying; YANG Hai-feng

doi:10.12452/j.fxcsxb.23061302

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In Situ Synthesis of Au/Foam Nickel Magnetic Substrate for Rapid Detection of Immunosuppressive Drug Metabolites in Serum by Using Surface Enhanced Raman Scattering Spectroscopy

Scientific Papers | 更新时间：2024-01-17

- In Situ Synthesis of Au/Foam Nickel Magnetic Substrate for Rapid Detection of Immunosuppressive Drug Metabolites in Serum by Using Surface Enhanced Raman Scattering Spectroscopy
- Journal of Instrumental Analysis Vol. 43, Issue 1, Pages: 200-206(2024)
- 作者机构：
  
  上海师范大学化学与材料科学学院，上海 200234
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.23061302
  CLC： O657.37;R318
- Published：15 January 2024，
  
  Received：13 June 2023，
  
  Revised：24 August 2023，
- 稿件说明：
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刘尧尧,李朵朵,郭小玉等.原位合成Au/泡沫镍磁性基底用于表面增强拉曼快速检测血清中免疫抑制剂代谢物[J].分析测试学报,2024,43(01):200-206.

LIU Yao-yao,LI Duo-duo,GUO Xiao-yu,et al.In Situ Synthesis of Au/Foam Nickel Magnetic Substrate for Rapid Detection of Immunosuppressive Drug Metabolites in Serum by Using Surface Enhanced Raman Scattering Spectroscopy[J].Journal of Instrumental Analysis,2024,43(01):200-206.
刘尧尧,李朵朵,郭小玉等.原位合成Au/泡沫镍磁性基底用于表面增强拉曼快速检测血清中免疫抑制剂代谢物[J].分析测试学报,2024,43(01):200-206. DOI： 10.12452/j.fxcsxb.23061302.

LIU Yao-yao,LI Duo-duo,GUO Xiao-yu,et al.In Situ Synthesis of Au/Foam Nickel Magnetic Substrate for Rapid Detection of Immunosuppressive Drug Metabolites in Serum by Using Surface Enhanced Raman Scattering Spectroscopy[J].Journal of Instrumental Analysis,2024,43(01):200-206. DOI： 10.12452/j.fxcsxb.23061302.

摘要

该文采用处理后的泡沫镍为反应模板，直接还原氯金酸溶液，经实验条件优化，原位制备了纳米金-泡沫镍多孔磁性复合表面增强拉曼散射（SERS）基底（Au-NFs）。泡沫镍三维网状具有大的比表面积和粗糙度，可稳定负载纳米金，通过Au—S键化学吸附更多的目标分子6-巯基嘌呤。复合基底的磁特性易于磁分离富集，避免血液共存物对SERS检测6-巯基嘌呤的干扰。利用磁场诱导纳米金-泡沫镍聚集，可进一步优化纳米金局域等离子场分布，形成SERS热点，大大提升了分析灵敏度，对于探针分子4-巯基吡啶的检出限可达5×10

-9

mol/L。在外磁场优化条件下，基于Au-NFs复合SERS基底定量检测6-巯基嘌呤，在5×10

-7

~1×10

-8

mol/L浓度范围内有良好的线性，检出限为1×10

-9

mol/L。该Au-NFs基底无需样品预处理，可实现血清中6-巯基嘌呤的快速分析，加标回收率在92.8%~107%范围内，表明方法可靠。

Abstract

In this paper，porous magnetic gold nano-foam nickel composite is in situ prepared as surface enhanced Raman scattering（SERS） substrate（Au-NFs） by directly reducing chloric acid gold solution with treated foam nickel as a reductive reaction template. The three-dimensional network of foam nickel with a large specific surface area and roughness could tightly load gold nanoparticles and chemically adsorb more target molecules 6-mercaptopurine via the Au—S bond. The magnetic properties of the composite substrate make it easy for magnetic separation and enrichment to avoid the interference of blood coexistence species on SERS detection of 6-mercaptopurine. By using magnetic field，porous magnetic Au-NFs can be further optimized the distribution of the local plasma field of gold nanoparticles to form SERS hot spots，which greatly improve the detection sensitivity. For probe molecule 4-mercaptopyridine，the Au-NFs-based SERS detection limit at 5×10

-9

mol/L could be reached. Under external magnetic field，with Au-NFs composite substrate，a linear detection of 6-mercaptopurine concentration locates in the range of 5×10

-7

and 1×10

-8

mol/L，with a detection limit of 1×10

-9

mol/L. Without sample pretreatment，this Au-NFs substrate could be used for rapid analysis of 6-mercaptopurine spiked in serum with recoveries ranging from 92.8% to 107%，indicating the reliability of the method.

关键词

泡沫镍金纳米磁复合材料6-巯基嘌呤SERS检测血清免疫抑制剂

Keywords

nickel foamAu nanoparticlesmagnetic composites6-mercaptopurineSERS detectionserumimmunosuppressive drug

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