Stroke is one of the leading causes for death and disability in the world,while ischemic stroke(IS) constitutes around 87 percent of stroke cases.Medical treatment should typically be made within 3 or 45 hours of stroke onset.Rapid diagnosis and early screening on IS are thus of great significance,and molecular biomarker is an effective strategy for this.Mass spectrometry is a choice of technique for detection of various compounds in biomedical samples,and has played important roles in discovery of biomarkers for IS.In this review,the recent advances in discovering biomarkers of IS,particularly those of the protein and metabolite biomarkers,are summarized and commented.