Comprehensive Structural Characterization of Unsaturated Lipids via Paternò-Büchi(PB) Reactions and Tandem Mass Spectrometry

您当前的位置：

首页 >

文章列表页 >

Comprehensive Structural Characterization of Unsaturated Lipids via Paternò-Büchi(PB) Reactions and Tandem Mass Spectrometry

更新时间：2023-05-25

- Comprehensive Structural Characterization of Unsaturated Lipids via Paternò-Büchi(PB) Reactions and Tandem Mass Spectrometry
- Vol. 39, Issue 1, Pages: 19-27(2020)
- 作者机构：
  
  1. 清华大学精密仪器系精密测试技术及仪器国家重点实验室
  2. ,北京,100084
  3. 清华大学化学系
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
Comprehensive Structural Characterization of Unsaturated Lipids via Paternò-Büchi(PB) Reactions and Tandem Mass Spectrometry. [J]. 39(1):19-27(2020)
DOI：

Comprehensive Structural Characterization of Unsaturated Lipids via Paternò-Büchi(PB) Reactions and Tandem Mass Spectrometry. [J]. 39(1):19-27(2020) DOI：

摘要

脂质在一系列关键生命过程中发挥着重要作用。结构决定功能，因此脂质结构解析与功能研究一直受到研究者的关注。尽管质谱已成为脂质分析的最常用技术之一，但脂质的精细结构解析仍面临诸多挑战。为解决上述问题，将Paternò-Büchi(PB)光化学反应与串联质谱相结合，实现了不饱和脂质中碳碳双键(C‖C)的定位以及不饱和脂质C‖C位置异构体的定量分析。此外，该技术可与鸟枪法、液相色谱-质谱和质谱成像等技术联用，用于复杂生物样品中脂质的分析。

Abstract

Lipids play important roles in a variety of critical biological processes.Therefore,significant research efforts have been devoted to the structural characterization and functional analysis of lipids.Although mass spectrometry(MS) has become one of the most common techniques for rapid and sensitive analysis of lipids,the structural characterization on lipids remains to be an analytical challenge.Aiming to solve this problem,an effective and widely adopted method was developed for localizing C‖C(s) in unsaturated lipids and quantifying lipid C‖C location isomers.Besides,this method could be readily coupled with shotgun lipidomics,liquid chromatography-mass spectrometry(LC-MS) and MS imaging,to achieve detailed lipid structural analysis on various types of biological samples.

关键词

脂质串联质谱光化学衍生结构解析

Keywords

lipidstandem mass spectrometryphotochemical derivatizationstructural elucidation

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Simultaneous Determination of Nicotine and Its Nine Metabolites in Human Urine Using Hydrophilic Interaction High Performance Liquid Chromatography－Tandem Mass Spectrometry

Research Advances in Precise Structural Characterization of Lipids by Ambient Ionization Mass Spectrometry

Determination of Chlormequat and Mepiquat Residues in Food by High Performance Liquid Chromatography-Tandem Mass Spectrometry

Simultaneous Determination of 5 Water-soluble Vitamins by HPLC with On-line Photochemical Derivatization and Fluorescence Detection

Determination of the Active Components in Flos Lonicerae by Microwave Assistant Extraction Combined with High Performance Liquid Chromatography-Tandem Mass Spectrometry

Related Author

No data

Related Institution

Zhengzhou Tobacco Research Institute of CNTC

广东工业大学生物医药学院

中山大学生命科学学院

农业部食品质量监督检验测试中心

厦门大学近海海洋环境科学国家重点实验室

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰