Detection of Glucose Using an Electrochemiluminescence Biosensor Based on Ru（bpy）2+3-Triethylamine System

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Detection of Glucose Using an Electrochemiluminescence Biosensor Based on Ru（bpy）2+3-Triethylamine System

更新时间：2023-05-25

- Detection of Glucose Using an Electrochemiluminescence Biosensor Based on Ru（bpy）2+3-Triethylamine System
- Vol. 39, Issue 3, Pages: 330-336(2020)
- 作者机构：
  
  1. 重庆市沙坪坝区人民医院消化内科
  2. 西南大学化学化工学院
- 作者简介：
- 基金信息：
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Detection of Glucose Using an Electrochemiluminescence Biosensor Based on Ru（bpy）2+3-Triethylamine System. [J]. 39(3):330-336(2020)
DOI：

Detection of Glucose Using an Electrochemiluminescence Biosensor Based on Ru（bpy）2+3-Triethylamine System. [J]. 39(3):330-336(2020) DOI：

摘要

联吡啶钌（Ru（bpy）2+3）拥有优良的电致化学发光（ECL）性能，但其较好的水溶性使其固载面临巨大问题。该文制备了Pt纳米粒子与Ru（bpy）2+3的复合物（Pt NPs-Ru），将其修饰于电极并进一步固载葡萄糖氧化酶（GOx）制得传感器。基于H2O2对Ru（bpy）2+3-三乙胺体系ECL信号的猝灭作用，随着葡萄糖浓度的增加，其在GOx的催化下原位产生的H2O2量增多，导致ECL信号逐渐减弱，从而实现葡萄糖的检测。ECL强度与葡萄糖浓度的对数在10×10-8~5.0×10-5 mol/L范围内呈良好的线性关系，检出限低至5.2×10-9 mol/L。传感器具有好的稳定性和高的选择性。Pt NPs-Ru复合物为ECL传感器的构建提供了良好平台，为葡萄糖检测提供了新方法。

Abstract

Ru（bpy）2+3 has an excellent electrochemiluminescence（ECL） properties.However,the good solubility makes it meet a huge problem with the immobilization.In this experiment,an ECL biosensor for detection of glucose was fabricated by modifying Pt NPs-Ru complexes composed of Pt nanoparticles and Ru（bpy）2+3 onto the electrode surface for immobilization of glucose oxidase（GOx）.In fact,the ECL signal of Ru（bpy）2+3-triethylamine system could be efficiently quenched by H2O2.With increase of glucose concentration,amount of H2O2 generated in situ by glucose under the catalysis of GOx increased,and the ECL signal gradually decreased,thus achieving the detection of glucose.A good linear relationship between ECL intensity and the logarithm of glucose concentration in the range of 1.0×10-8-5.0×10-5 mol/L was obtained,with a detection limit of 5.2×10-9 mol/L.The biosensor showed good stability and high selectivity.The Pt NPs-Ru complexes provide a good platform for construction of ECL sensor and a new method for detection of glucose.

关键词

电致化学发光（ECL）葡萄糖生物传感器Ru（bpy）2+3

Keywords

electrochemiluminescence（ECL）glucosebiosensorRu（bpy）2+3

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Related Institution

College of Chemical Engineering，Fuzhou University

College of Materials and Chemical Engineering，Minjiang University

重庆市第八中学校

聊城大学化学化工学院

Hunan Provincial Key Laboratory of Water Treatment Functional Materials，Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology，College of Chemistry and Materials Engineering，Hunan University of Arts and Science

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