A fluorescent method based on copper/silver nanoclusters for the detection of Cu2+ was established by designing DNA sequences with G rich bases and investigating the fluorescence enhancement effect of G base pairs on DNA-Cu/AgNCs.It was found that the fluorescence intensity of the prepared DNA-Cu/AgNCs was significantly enhanced by the G5 sequence at the 5′ end of the C rich sequence template.Meanwhile,the fluorescence of DNA-Cu/AgNCs was quenched by Hg2+ and Cu2+.NaBH4 could be used to mask Hg2+ in order to achieve a selective detection of Cu2+.The linear range of this method for Cu2+ was from 0.01 μmol/L to 5.0 μmol/L,and the detection limit was 5.0 nmol/L.The method is simple,rapid,highly selective and low cost,and is able to apply in detection for real samples.
Research Progress of Design,Synthesis and Application of Fluorescent Nanoprobe
Determination of Copper Ion by Electrochemiluminescence Using Graphene Quantum Dots Doped with Nitrogen and Sulfur
Recent Advances in Metal Nanoclusters-based Fluorescent Platforms for Biosensing
Investigation on Effect of Fluorescence Enhancement of Danshensu-BSA System by Synchronous Fluorescence Spectrometry
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Institute of Medical Support Technology,Academy of System Engineering of Academy of Military Sciences
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; Shandong Key Laboratory of Biochemical Analysis; College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology