A novel high performance liquid chromatography(HPLC) with magnetic solid phase extraction(MSPE) based on multi template molecularly imprinted polymer(MIP) was developed for the detection of tetracyclines in environmental water samples.The multi template MIP was prepared by sol-gel method,with mesoporous silicon magnetic graphene oxide as carrier,tetracycline,oxytetracycline,chlortetracycline and doxycycline as templates,and N-［3-(trimethoxysilyl)propyl］ethylenedi amine(KH-792) and anilinomethyl triethoxysilane(KH-42) as functional monomers,which was characterized by field emission scanning electron microscopy(SEM),infrared spectroscopy(IR) and vibrating sample magnetometry(VSM).The method was applied in the detection of tetracycline,oxytetracycline,chlortetracycline and doxycycline in water samples.Results showed that the calibration curves for 4 tetracyclines were linear in the range of 5-50 μg/L,with their detection limits and quantitation limits of 0.67-0.95 μg/L and 2.13-3.50 μg/L,respectively.The spiked recoveries ranged from 82.7% to 103% with relative standard deviations(RSD) of 1.0%-8.8%.The proposed method could be used for the simultaneous and selective detection of these four tetracyclines in real environmental water samples.