In this work,an ultra high performance liquid chromatography-quadrupole-orbitrap mass spectrometry was developed for non directional screening of mycotoxins in dairy products.Under the mass spectrometry data dependent scanning mode,the molecular ion mass to charge ratios,secondary fragmented fragments mass to charge ratios and retention times of 38 typical mycotoxins reference materials were collected.A standard database for mycotoxins was established,and the analysis on fragmentation path was completed.The characteristic fragment fragments were combined with the standard database for mycotoxins in a mass spectrum independent scanning mode to finally achieve a non-directional screening of mycotoxins in dairy products.The detection limit(CCα) and the detection capacity(CCβ) of this method were in the ranges of 0.01-0.36 μg/kg and 0.03-0.57 μg/kg,respectively.and the recoveries ranged from 81.9% to 111% with relative standard deviations of 1.1%-6.1%.The established method was used to screen 40 batches of pasteurized milk,ultra high temperature instant sterilized milk,milk containing beverages and infant formula milk powder.Results showed that the method was simple and rapid,and could meet the requirements for accurate determination and quantitation of mycotoxins in dairy products.