In this study,a molecularly imprinted electrochemical sensor for tetracycline（TC）,MIPs/GQDs-AuNPs/GCE was constructed,with TC as template molecule and 4-aminothiophenol（4-ATP） as functional monomer,by electrically polymerizing them both on the surface of glassy carbon electrode modified with gold nanoparticles and graphene quantum dot composites through cyclic voltammetry（CV）.CV,electrochemical impedance spectroscopy（EIS） and linear scan voltammetry（LSV） were used to investigate the electrochemical response of the molecularly imprinted sensor.Results showed that the sensor possessed a good current response to tetracycline.Under the best experimental conditions,there was a good linear relationship for the oxidation peak current of TC in the concentration range of 2.0×10-8-3.0×10-5 mol/L,with a correlation coefficient of 0.999 4.The detection limit was 1.5×10-9 mol/L.The recoveries were in the range of 97.9%-106%.With good stability,high sensitivity and selectivity,the sensor has a good prospect for application.