A two dimensional thin layer chromatography-surface enhanced Raman scattering(TLC-SERS) method was constructed with highly stable and sensitive nanogold/porous silica composites as SERS substrate.This technique improved the separation capability by combining the rapid separation function of TLC and the qualitative and quantitative analysis function of SERS.The homogeneity and batch reproducibility of the substrate were studied using a p-aminothiophenol as probe.Mapping experiment showed that the substrate had a good point-point repeatability,with a relative standard deviation(RSD) of 5.9% for the peak intensities at 24 measuring points.Moreover,the RSD for 9 batches of substrates was 75%.The linear range of the substrate for the detection of saccharin sodium was 0.1-200 mg/L with the detection limit of 0.06 mg/L.The substrate was applied in the detection of saccharin sodium in actual samples,with the target concentrations in beverage and fruit of 141 mg/L and 18 mg/L,respectively.The whole analytical process could be completed within 10 min.With the advantages of high sensitivity,strong specificity and simple pretreatment,the method could realize the rapid determination of saccharin sodium in drinks and fruits.