An ultra high performance liquid chromatography-tandem mass spectrometry with solid phase extraction was developed for the determination of bisphenol A(BPA)，bisphenol F(BPF)，tetrachlorobisphenol A(TCBPA)，tetrabromobisphenol A(TBBPA)，4 nonylphenol(NP)，4-octylphenol(4-n-OP) in human urine.Human urine sample was purified by solid phase extraction after glucuronidase,then separated on an Acquity UPLC HSS T3 column(2.1 mm×100 mm,1.8 μm).The 6 analytes were determined in negative electrospray(ESI-) under multiple reaction monitoring(MRM) mode,and quantified by isotope labeled internal standard method.There were good linear relationships for 6 analytes in the range of 0.5-50 μg/L with their correlation coefficients larger than 0.995.The limits of detection and the limits of quantitation were in the ranges of 0.05-0.60 μg/L and 0.17-2.00 μg/L,respectively.Recoveries at three spiked levels of 2,10 and 50 μg/L ranged from 81.4% to 112% with relative standard deviations(RSD) of 6.8%-30%.The method was utilized in the determination of 160 human urine samples with a detection rate of 93.8% for BPA in the range of 0.24-2954 μg/L,and other substances were not detected.With the advantages of convenient operation,good accuracy and stability,this method is suitable for the determination of bisphenols and alkylphenols in human urine.