A portable microplate analyzer based on a smartphone was developed for rapid high throughput quantitative detection of human serum albumin(HSA).A lens with large aperture and a LED with uniform plate were firstly used in this article to assist the smartphone to achieve a uniform imaging for the whole 96 microplate.A custom designed smartphone application was used for automatic analysis.There was a good linear relationship between results obtained with this analyzer and those of microplate reader for detection of HSA standard samples,with a correlation coefficient of 0.989 3 and the detection range of 21.45-60.06 g/L,which proved that the developed analyzer has great accuracy and reliability.In addition,the detection results of human serum samples also showed good agreement between the clinical analyzer and the proposed one,revealing that the developed analyzer could also be used for clinical HAS detection.With the characteristics of low cost and being small in size,the analyzer is suitable for those underdeveloped areas,primary care institutions and ordinary families,and is of great significance for rapid on site diagnosis on the disease.