Study on Identification Method for Caffeoylquinic Acids Isomers Based on Liquid Chromatography-Tandem Mass Spectrometry

您当前的位置：

首页 >

文章列表页 >

Study on Identification Method for Caffeoylquinic Acids Isomers Based on Liquid Chromatography-Tandem Mass Spectrometry

更新时间：2023-04-18

- Study on Identification Method for Caffeoylquinic Acids Isomers Based on Liquid Chromatography-Tandem Mass Spectrometry
- Vol. 39, Issue 4, Pages: 461-466(2020)
- 作者机构：
  
  1. 齐鲁工业大学(山东省科学院)
  2. 山东省分析测试中心
  3. 山东省中药质量控制技术重点实验室
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
Study on Identification Method for Caffeoylquinic Acids Isomers Based on Liquid Chromatography-Tandem Mass Spectrometry. [J]. 39(4):461-466(2020)
DOI：

Study on Identification Method for Caffeoylquinic Acids Isomers Based on Liquid Chromatography-Tandem Mass Spectrometry. [J]. 39(4):461-466(2020) DOI：

摘要

该文对单咖啡酰奎宁酸和二咖啡酰奎宁酸的位置异构体分别进行液相色谱分析和不同碰撞能下的二级质谱分析，并对其质谱裂解规律进行了研究。结果发现，单咖啡酰奎宁酸的母离子377和子离子163在不同碰撞能下其强度发生显著变化，通过377/163的强度比值可区分其位置异构体。377/163比值大小依次为3-O-咖啡酰奎宁酸、4-O-咖啡酰奎宁酸、5-O-咖啡酰奎宁酸。二咖啡酰奎宁酸的母离子539和子离子377、163在不同碰撞能下其强度发生显著变化，通过539/377、377/163的强度比值可区分其位置异构体。539/377比值大小依次为4,5-O-二咖啡酰奎宁酸、3,4-O-二咖啡酰奎宁酸、3,5-O-二咖啡酰奎宁酸。377/163比值大小依次为3,5-O-二咖啡酰奎宁酸、4,5-O-二咖啡酰奎宁酸、3,4-O-二咖啡酰奎宁酸。基于Agilent Poroshell 120 SB-Aq C18色谱柱，不同梯度下咖啡酰奎宁酸位置异构体的洗脱顺序均为5-O-咖啡酰奎宁酸、4-O-咖啡酰奎宁酸、3-O-咖啡酰奎宁酸、3,4-O-二咖啡酰奎宁酸、3,5-O-二咖啡酰奎宁酸和4,5-O-二咖啡酰奎宁酸。通过色谱保留特征和质谱裂解规律对金银花中咖啡酰奎宁酸的位置异构体实现了准确鉴别。

Abstract

In this paper,the positional isomers of monocaffeoylquinic acid and dicaffeoylquinic acid were separated and analyzed by liquid chromatography(LC),then analyzed by tandem mass spectrometry(MS/MS) with different collision energies.The fragmentation rules for the analytes were investigated using MS/MS.For the positional isomers of monocaffeoylquinic acid,as the intensities of their parent ion 377 and product ion 163 changed significantly under different collision energies,they could be distinguished by the intensity ratios of 377/163.The isomers with intensity ratios of 377/163 in turn were 3-O-caffeoylquinic acid,4-O-caffeoylquinic acid and 5-O-caffeoylquinic acid.As for the positional isomers of dicaffeoylquinic acid,the intensities of their parent ion 539 and product ions 377 and 163 changed significantly under different collision energies,they could be distinguished by their intensity ratios of 539/377 and 377/163.The isomers with intensity ratios of 539/377 in turn were 4,5-di-O-caffeoylquinic acid,3,4-di-O-caffeoylquinic acid and 3,5-di-O-caffeoylquinic acid,while the isomers with intensity ratios of 377/163 in order were 3,5-di-O-caffeoylquinic acid,4,5-di-O-caffeoylquinic acid and 3,4-di-O-caffeoylquinic acid.The positional isomers of caffeoquinic acid were separated and analyzed based on an Agilent Poroshell 120 SB-Aq C18 column,and the eluting orders were as follows:5-O-caffeoylquinic acid,4-O-caffeoylquinic acid,3-O-caffeoylquinic acid,3,4-di-O-caffeoylquinic acid,3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid.The positional isomers of caffeoylquinic acid in honeysuckle were identified accurately based on the chromatographic retention and MS/MS characteristics.

关键词

单咖啡酰奎宁酸二咖啡酰奎宁酸位置异构体液相色谱-串联质谱(LC-MS/MS)离子强度比

Keywords

monocaffeoylquinic aciddicaffeoylquinic acidpositional isomersliquid chromatography-tandem mass spectrometry(LC-MS/MS)ion intensity ratio

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Separation of Positional Isomers Using Core-shell Composite NH₂－MIL－125@TPA－COF Column with High Performance Liquid Chromatography

Scientific PapersDetermination of 27 Novel Fentanyl Analogs and Metabolites in Solid and Liquid Drugs by Liquid Chromatography-Tandem Mass Spectrometry

Preparation and Evaluation of a Chiral Stationary Phases Based on Poly L-Glutamic Acid Ethyl Ester in HPLC

Simultaneous Determination of Six Pyrazole Fungicides in Grape Wine by Liquid Chromatography-Tandem Mass Spectrometry

Simultaneous Determination of 14 Antipsychotic and Sedative Substances in Human Nails Using Microwave-assisted Extraction Combined with Liquid Chromatography-Tandem Mass Spectrometry

Related Author

No data

Related Institution

Department of Chemistry and Chemical Engineering，Yunnan Normal University

深圳海关食品检验检疫技术中心

云南师范大学化学化工学院

温州出入境检验检疫局

温州大学化学与材料工程学院

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰