A time-resolved immunofluorescence chromatography was established for the rapid and sensitive evaluation of SARS-CoV-2 antibody level in patients suffering Corona Virus Disease 2019,with a truncated severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) N protein and a full-length SARS-CoV-2 N protein.The test strip cards were prepared with fluorescent microspheres as markers,which were assembled for negative and positive detection to pick out the superior antigen pairing combination.Meanwhile,the precision and stability of the optimized test strip card were further evaluated for preliminary performance,and compared with those of the marketed SARS-CoV-2 antibody detection method on this basis.According to the negative and positive detection results,preferably,the truncated N protein was labeled and the full-length N protein was coated.This method could complete the detection of SARS-CoV-2 antibody level within 15 minutes,and could effectively distinguish the negative and positive results.The intra-assay precision CV was between 3.48% and 10.05%,and the inter-assay precision CV was between 4.77% and 11.73%.The strips could be stably stored at 37 ℃ for 21 days.The detection results by this method were well correlated with those by colloidal gold assay,with a total coincidence rate of 96.13%,a positive coincidence rate of 90.63% and a negative coincidence rate of 97.56%,respectively.The fluorescence immunochromatographic assay preliminarily established based on truncated N protein could effectively evaluate the SARS-CoV-2 antibody level in patients with good precision and stability,which was convenient for popularization in primary care units and had a good application prospect.