Gel permeation chromatography(GPC) is a separation technology developed in the 1960s,which has the advantages of low requirements for mobile phase,mild experimental conditions,high reproducibility,rapid analysis and high solute recovery,etc.As a kind of liquid separation chromatography,gel permeation chromatography has a unique separation effect,it has been rapidly developed and widely used in many fields such as petrochemical engineering,biomedicine,food hygiene,environmental monitoring,etc.Progresses in the synthesis,performance and application of column fillers for gel permeation chromatography such as polyacrylamide gel and polystyrene gel in recent years are reviewed in this article,and the new fillers,as well as their application fields and prospects are also introduced.