The accurate and rapid quantitative detection of biomarkers has a significant influence on controlling and preventing disease or toxin outbreaks.In this study,3D flower like Au@(MoS2/GO/o MWNTs) nanohybrids(AMGMs) were introduced as a desirable substrate for sensitive electrochemiluminescence(ECL) detection of prostate specific antigen(PSA).Specifically,the AMGMs acted as a substrate material with desirable electrical conductivity,excellent biocompatibility and a large specific surface area for loading abundant primary antibody,which could gather abundant amounts of Ru complexes on the electrode,giving an amplified signal.The assembly process of this immunosensor was investigated by electrochemical impedance spectroscopy(EIS) and cyclic voltammetry(CV),and confirmed by ECL method.The proposed ECL immunosensor was applied in the detection on different contents of PSA in the substrate,with a wide linear range of 01 pg/mL-50 ng/mL and a detection limit(S/N=3) of 01 pg/mL.The spiked recoveries for actual samples ranged from 982%to 106%.The proposed method exhibited the advantages of excellent stability and selectivity in determination of PSA antigen,providing a sensitive and novel ECL analysis platform for the trace detection of other biomarkers.