Liquid crystal(LC) biosensors develop based on highly sensitive responses of LC to interfacial properties and its inherent optical anisotropy,which show extraordinary application value in detecting and analyzing of biological samples.By decorating with a stimuli-responsive molecule,LC interface can sensitively respond to the presence of biological analytes,which directs the orientation of LC molecules at the LC interface.Further,the short-range interaction occurred at the LC interface can change the orientation of bulk LC phase and lead to a visual transfiguration through a polarized optical microscope(POM).LC biosensors does not need to label the target analyte,and easily transforms molecular events into detectable optical signals with a simple optical instrument.Because of the advantages of simplicity,sensitivity,efficiency,rapidity and low cost,LC biosensors have been widely used in the field of bioanalysis.Herein,this article briefly reviews the recent advances in LC biosensors for the analysis of small biomolecules,biomacromolecules and organism.In particular,we discuss the development and challenge of the emerging area where the LC biosensor is introduced to a microfluidic cell chip.LC biosensors have been successfully applied to cell analysis with complex and diverse interfacial phenomena.In the future,the design and usability of LC sensors in the simulated cell microenvironment need to be further studied.