A new cyclodextrin derivative heptakis(26 di O ethoxyethyl 3 O trifluoroacetyl) β cyclodextrin was synthesized by substituting the 26 OH groups of cyclodextrin derivative with ethoxyethyl groups and the 3 OH group with trifluoroacetyl group,and used to coat capillary column of gas chromatography by static method.The chromatographic properties and separation abilities of the capillary column were studied.The results showed that the stationary phase possessed good separation efficiencies to Grob test mixture,disubstituted benzene isomers(such as chlorotoluene,nitrotoluene and bromotoluene) and ten kinds of chiral compounds(such as α substituted propionate,1 (2′ nitrophenyl) ethanol,α methyl 4 chlorobenzyl cyanide,2 methyl 4 oxo 3 prop 2 ynyl cyclopent 2 enyl acetate).Among the test compounds,the separation ability of chiral stationary phase for 2 methylsulfonylpropionate was the best,the separation efficiency of methyl ester derivatives of chiral stationary phase for α substituted propionic was better than that of ethyl ester derivatives,and methyl ester and ethyl ester derivatives of α hydroxypropionic were better than that of α halogenpropionic.