Enantioseparation of amlodipine besylate on Chiralcel OJ chiral column was studied.The influences of different carbon number，alcohol modifier types with different three dimensional structures and concentrations,flow rate and column temperature on the enantioseparation of amlodipine besylate were investigated.And the chiral recognition mechanism for amlodipine was discussed by the chromatographic retention and thermodynamic parameters.A good baseline resolution was obtained for the separation of amlodipine besylate using hexane-isopropanol（90∶10,by volume） as mobile phase with flow rate of 04 mL/min and column temperature of 20 ℃.The resolution of amlodipine was up to 3228.In the experimental temperature range，Van′t Hoff curve with a good linearity was acquired by ploting lnα to 1/T.The enthalpy change difference，entropy change difference and Tiso of the enantioseparation on chiral column were calculated.The enantioseparation meeting the requirements of ‖ΔΔH0‖>‖ΔΔS0‖ showed that the process of chiral sepatation was an enthalpy controlled process.