Determination of Buddleoside by Capillary Electrophoresis

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Determination of Buddleoside by Capillary Electrophoresis

- Determination of Buddleoside by Capillary Electrophoresis
- Vol. 30, Issue 2, Pages: 225-228(2011)
- 作者机构：
  
  1. 绵阳师范学院化学与化学工程学院
  2. 中国空气动力研究与发展中心设备设计及测试技术研究所
  3. 中山大学化学与化学工程学院
- 作者简介：
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Determination of Buddleoside by Capillary Electrophoresis. [J]. 30(2):225-228(2011)
DOI：

Determination of Buddleoside by Capillary Electrophoresis. [J]. 30(2):225-228(2011) DOI：

摘要

使用自行设计的毛细管电泳柱端安培检测系统，采用非水介质毛细管电泳法对野菊花中蒙花苷的含量进行测定。考察了缓冲溶液浓度及其pH值、非水介质的浓度、检测电位对测定的影响。在优化实验条件下，蒙花苷的质量浓度在20～80 mg/L范围内与峰高呈良好的线性关系，线性系数为0999 6，检出限为020 mg/L，平均加标回收率为99%。

Abstract

Using self constructed electrochemical detection system，buddleoside was detected by high performance capillary electrophoresis(HPCE).Several parameters affecting the determination were studied，including effects of potential of working electrode，organic concentration,running buffer，acidity and working voltage.Under the optimal conditions，a linear relationship between the peak height（Y） and the mass concentration（c） was obtained in the range of 20-80 mg/L with a correlation coefficient of 0999 6.The determination limit was 02 mg/L and the average recovery was 99%.

关键词

毛细管电泳蒙花苷安培检测

Keywords

capillary electrophoresisbuddleosideamperometric detection

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绵阳师范学院化学系

中国空气动力研究与发展中心设备设计及测试,3.中山大学化学与化学工程学院

School of Environmental and Pharmaceutical Engineering，Taizhou Institute of Science and Technology，Nanjing University of Science and Technology

Jiangsu Key Laboratory of Environmental Science and Engineering

School of Environmental Science and Engineering，Suzhou University of Science and Technology

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