In this paper,the evaluation of moisture，total acid(lactic acid) and amino acid in Fuling mustard tuber by Fourier transform near infrared spectroscopy(FT-NIRS)was studied.The original spectra of 58 calibration samples of Fuling mustard tuber were obtained using FT-NIRS machine,and processed with math analysis such as first derivative,straight line subtraction,multiplicative scattering correction and min-max normalization to decrease the noise.The partial least squares(PLS) calibration models were established under the optimal conditions to predict the content of moisture，total acid(lactic acid) and amino acid.Determination coefficient(R2),root mean square error of cross validation(RMSECV) and root mean square error of prediction(RMSEP) were used to evaluate the quality of the model.The R2 and RMSECV of the model for moisture,total acid and amino acid were 0957 8,0975 4,0950 4,and 0107，0017 1，0036 3,respectively,while the R2 and RMSEP for them three were 0956 2,0953 9,0946 0 and 0256，0034 7，0038 8 in test set validation,respectively.By statistical significance test,the determination results were compared with those of standard methods without significant difference at 005 level.Internal and external cross certification test showed that near infrared quantitative analysis could meet the requirement of prediction of moisture，total acid and amino acid content of Fuling mustard tuber in production with high accuracy.