Determination of trace dopamine has important significance on physiology，disease diagnosis and pharmaceuticals quality. In acid medium and surfactant，the fluorescence intensity of acridine orange could be quenched in the presence of graphene oxide.However,when dopamine was added into the system solution，the fluorescence intensity of the system was increased and the increasing fluorescence intensity was proportional to the amount of dopamine.Based on this,a novel method for the determination of dopamine was developed.The calibration curve was linear in the range of 0.05-12.0 μmol/L.The linear regression equation was ΔIF= 3.9+57.8c（μmol/L） （r=0.993 3） and the detection limit was 2.9 nmol/L. The method showed high sensitivity，and was successfully applied in the determination of dopamine in real samples with satisfactory results.