A novel method was developed for the qualitative and quantitative analysis of trace volatile organic compounds(VOCs）,methyl jasmonate and methyl salicylate,emitted from peanut based on a designed dynamic adsorption device coupled with high performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS）.The VOCs were adsorbed with Tenax TA,eluted with methanol solution containing 0.1% formic acid,and concentrated with nitrogen.The separation of the VOCs was carried out on an Agilent C18 column by gradient elution mode using methanol-005% formic acid as mobile phase at a flow rate of 300 μL/min.The samples were analyzed by ion trap mass spectrometer under positive ion mode with multiple reaction monitoring.The internal standard method was used for quantitative analysis.The detection limits(S/N=3） for methyl jasmonate and methyl salicylate were 2.0 μg/L and 20.0 μg/L,and the quantitation limits(S/N=10）were 5.0 μg/L and 50.0 μg/L,respectively.There was a good linear relationship(r>0.98） between the concentration and the peak area ratio in the range of 1.0-500 μg/L for methyl jasmonate and 20-1 000 μg/L for methyl salicylateThe method was simple,rapid and high efficient,and was suitable for the investigation of changes of volatile organic compounds emitted from plant at different treatment conditions.