Preparative high-speed counter-current chromatography(HSCCC) and high performance liquid chromatography(HPLC) were employed firstly to separate chemical compounds in Vitex negundo L.. The procedure was performed in a two phase solvent system of chloroform-methanol-water (4∶3∶2,by volume) at a flow rate of 2.0 mL?min-1 by normal phase elution,in which the upper phase was the stationary phase and the lower phase was the mobile phase.The sample concentration was 20 g?L-1,while the rotation speed was 850 r?min-1 and the eluate was detected at 254 nm.Five compounds were obtained from the crude extract of the plant in a simultaneous process,which,according to their physical and chemical properties,were identified as:vitexin(Ⅰ),casticin(Ⅱ),3,5-di-O-caffeylquinic acid(Ⅵ),luteolin(Ⅶ),luteolin-7-O-β-D-glucoside(Ⅷ).The purities of the five compounds were 98.0%,92.3%,96.8%,97.4%and 90.3%,respectively.
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Department of Chemistry and Chemical Engineering,Yunnan Normal University
School of Chemistry and Chemical Engineering,Lanzhou City University
Provincial Key Laboratory of Gansu Higher Education for City Environmental Pollution Control
Provincical Key Laboratory of Gansu Higher Education for City Environmental Pollution Control