Different extraction methods of bioactive component squalene in rices were studied and a new method was developed for the determination of squalene in rices.The squalene in rices from different areas were extracted by Soxhlet extract method,and analyzed by gas chromatography-tandem mass spectrometry（GC-MS/MS）.The results showed that the calibration curve was linear over the concentration of squalene in the range of 1-50 mg/L with a correlation coefficient of 0.998 7.The detection limit（LOD） was 0.1 mg/kg.The recoveries of squalene at two spiked levels of 5 mg/kg and 20 mg/kg were in the range of 96%-104% with relative standard deviations（RSDs） no more than 3.6%.The method was sensitive and reproducible,and could be used to distinguish effectively rices with different species,origins and qualities.