A UPLC-MS/MS method was developed for the determination of 18 polyphenolic compounds in juice concentrate.The sample was diluted with water and extracted completely by an Oasis(HLB) SPE column.The extract was evaporated to dryness and redissolved with methanol-0.1% formic acid.The targeted compounds were separated on an Acquity UPLC- BEH C18（1.7 μm× 2.1 mm×50 mm） column using 0.1% formic acid-methanol as mobile phase by linear gradient elution at a flow rate of 0.3 mL?min-1,and detected by MS/MS.The quantification of eighteen polyphenolic compounds was performed by the external standard method.Under the optimal conditions,the calibration curves of 18 polyphenolic compounds were linear in the range of 10.0-10 000.0 μg?L-1 with detection limits of 10.0-1 000.0 ng?g-1.The average recoveries for all the analytes were more than 70% with RSDs（n=6） less than 8.0%.With the advantages of good reproducibility,good recovery and high sensitivity,the method was suitable for the analysis of polyphenolic compounds in juice concentrate.