A direct,simple and sensitive method was developed for the simultaneous separation and detection of nine organic acids,including lactate,acetate,propionate,formate,butyrate,malate,malonate,oxalate and citrate,in tobacco by anion exchange chromatography with eluent autogenerator and suppressed conductivity detection.The retention behaviors of organic acids on the anion exchange column were investigated using potassium hydroxide produced by EGC-ⅡKOH eluent autogenerator as eluent and the optimized gradient elution condition was obtained.The samples were prepared through extraction,filtration and dilution.The separation was performed on a Dionex IonPac AS11-HC anion exchange column.Under the optimized conditions,the calibration curves of all the analytes had good linearity.The detection limits ranged from 0.013 μg/L to 0.065 μg/L,and the relative standard deviations（RSDs） were in the range of 0.7%-3.0%.The spiked recoveries of nine organic acids were in the range of 93%-103%.The method was simple,accurate and sensitive，and could meet the actual requirements for the analysis of tobacco samples.