Different pretreatment methods（e.g.acetonitrile extraction，acetonitrile extraction-solid phase extraction（SPE），matrix solid phase dispersion（MSPD） extraction and MSPD-SPE） for the simultaneous analysis of penicillin,ampicillin and amoxicillin in chicken tissues were compared with HPCE.The results indicated that,among these methods,the MSPD method was of high precision,high recovery,easy operation and good practicability.Under the optimal chromatographic conditions,the calibration curves were linear in the range of 100-1 000 μg／L for penicillin and ampicillin,and 50-1 000 μg/L for amoxicillin.At the spiked concentrations of 50,500,1 000 μg／kg，the recoveries of these three drugs in chicken tissues ranged from 73％ to 96％ with relative standard deviations（RSDs） of 2.9％-9.8％.The intra-RSDs and inter-RSDs ranged from 4.0％ to 12.0％.The detection limits of penicillin,ampicillin and amoxicillin were 20,30,10 μg／kg,respectively,and their quantitation limits were 50,50,40 μg／kg,respectively.The method could meet the requirements for the residue analysis in accuracy and precision.