A liquid chromatography tandem mass spectrometric（LC-MS/MS）method was developed for the simultaneous determination of T-2，zearalenone（ZEN） and deoxynivalenol（DON） in wheat.The sample was extracted with acetonitrile-water（80∶20）.The extract was purified with an Amino column（AccuBond-AMINO，500 mg，6 mL）.Three myctoxins were completely separated on a ZORBAX Extend-C18 column（150 mm×2.1 mm,1.8 μm）by gradient elution with methanol and 5 mmol/L ammonium acetate as mobile phase，and detected by positive ion electrospray ionization mass spectrometry（ESI MS） under multiple reaction monitoring（MRM） mode.The results showed that the linearities of T-2,ZEN and DON were in the concentration ranges of 0.5-500，5-500,10-2 000 μg/L，respectively，with correlation coefficients of 0.998 9，0.999 7 and 0.999 1，respectively.The spiked recoveries of T-2，ZEN and DON at three spiked levels in wheat were in the ranges of 86%-94%，80%-101% and 81%-105%，with relative standard deviations（RSDs，n=5） of 2.6%-5.5%，3.6%-8.9% and 2.2%-8.1%，respectively.The limits of detection for T-2，ZEN and DON were 0.5，8.0,10.0 μg/kg，respectively.The method was low cost，sensitive and accurate，and was suitable for the simultaneous determination of T-2,ZEN and DON in wheat.